9ITF

Cryo-EM structure of full-length phyB(Y276H)-PIF6beta complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (C, A)	Phytochrome B	polymer	1226	135782.5	2	UniProt (P14713) Pfam (PF08446) Pfam (PF01590) Pfam (PF00360) Pfam (PF00989) Pfam (PF00512) Pfam (PF02518)	Arabidopsis thaliana (thale cress)	Protein LONG HYPOCOTYL 3,Protein OUT OF PHASE 1
2	C (B)	Transcription factor PIF6	polymer	241	26650.3	1	UniProt (Q8L5W7)	Arabidopsis thaliana (thale cress)	Basic helix-loop-helix protein 132,AtbHLH132,bHLH 132,Phytochrome interacting factor 3-like 2,Phytochrome-interacting factor 6,Transcription factor EN 111,bHLH transcription factor bHLH132
3	D, E (C, A)	3-[5-[[(3~{R},4~{R})-3-ethyl-4-methyl-5-oxidanylidene-3,4-dihydropyrrol-2-yl]methyl]-2-[[5-[(4-ethyl-3-methyl-5-oxidanylidene-pyrrol-2-yl)methyl]-3-(3-hydroxy-3-oxopropyl)-4-methyl-1~{H}-pyrrol-2-yl]methyl]-4-methyl-1~{H}-pyrrol-3-yl]propanoic acid	non-polymer		586.7	2	Chemie (O6E)

Sequence modifications

C, A: 1 - 1172 (UniProt: P14713)

PDB	External Database	Details
Met -28	-	initiating methionine
Asp -27	-	expression tag
Tyr -26	-	expression tag
Lys -25	-	expression tag
Asp -24	-	expression tag
Asp -23	-	expression tag
Asp -22	-	expression tag
Asp -21	-	expression tag
Lys -20	-	expression tag
Gly -19	-	expression tag
Asp -18	-	expression tag
Tyr -17	-	expression tag
Lys -16	-	expression tag
Asp -15	-	expression tag
Asp -14	-	expression tag
Asp -13	-	expression tag
Asp -12	-	expression tag
Lys -11	-	expression tag
Ile -10	-	expression tag
Asp -9	-	expression tag
Tyr -8	-	expression tag
Lys -7	-	expression tag
Asp -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Lys -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
His 276	Tyr 276	engineered mutation
Lys 1173	-	expression tag
Leu 1174	-	expression tag
Gly 1175	-	expression tag
Pro 1176	-	expression tag
Glu 1177	-	expression tag
Gln 1178	-	expression tag
Lys 1179	-	expression tag
Leu 1180	-	expression tag
Ile 1181	-	expression tag
Ser 1182	-	expression tag
Glu 1183	-	expression tag
Glu 1184	-	expression tag
Asp 1185	-	expression tag
Leu 1186	-	expression tag
Asn 1187	-	expression tag
Ser 1188	-	expression tag
Ala 1189	-	expression tag
Val 1190	-	expression tag
Asp 1191	-	expression tag
His 1192	-	expression tag
His 1193	-	expression tag
His 1194	-	expression tag
His 1195	-	expression tag
His 1196	-	expression tag
His 1197	-	expression tag

B: 1 - 183 (UniProt: Q8L5W7)

PDB	External Database	Details
Met -26	-	initiating methionine
Gly -25	-	expression tag
Ser -24	-	expression tag
Ser -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
Ser -16	-	expression tag
Ser -15	-	expression tag
Gly -14	-	expression tag
Leu -13	-	expression tag
Val -12	-	expression tag
Pro -11	-	expression tag
Arg -10	-	expression tag
Gly -9	-	expression tag
Ser -8	-	expression tag
His -7	-	expression tag
Ser -6	-	expression tag
Asp -5	-	expression tag
Glu -4	-	expression tag
Val -3	-	expression tag
Asp -2	-	expression tag
Ala -1	-	expression tag
His 0	-	expression tag
Glu 184	-	expression tag
Ser 185	-	expression tag
Ala 186	-	expression tag
Trp 187	-	expression tag
Ser 188	-	expression tag
His 189	-	expression tag
Pro 190	-	expression tag
Gln 191	-	expression tag
Phe 192	-	expression tag
Glu 193	-	expression tag
Lys 194	-	expression tag
Gly 195	-	expression tag
Gly 196	-	expression tag
Gly 197	-	expression tag
Ser 198	-	expression tag
Gly 199	-	expression tag
Gly 200	-	expression tag
Gly 201	-	expression tag
Ser 202	-	expression tag
Gly 203	-	expression tag
Gly 204	-	expression tag
Ser 205	-	expression tag
Ala 206	-	expression tag
Trp 207	-	expression tag
Ser 208	-	expression tag
His 209	-	expression tag
Pro 210	-	expression tag
Gln 211	-	expression tag
Phe 212	-	expression tag
Glu 213	-	expression tag
Lys 214	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	298215.3
Non-Polymers*	Number of molecules	2
	Total formula weight	1173.4
All*	Total formula weight	299388.7

*Water molecules are not included.