9IP4

Cryo-EM structure of the RNA-dependent RNA polymerase complex from Marburg virus

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	RNA-directed RNA polymerase L,Maltose/maltodextrin-binding periplasmic protein	polymer	1851	210153.0	1	UniProt (P31352) UniProt (P0AEX9) Pfam (PF00946) Pfam (PF14318)	Marburg virus - Musoke Kenya 1980	Protein L,Large structural protein,Replicase,Transcriptase,MMBP,Maltodextrin-binding protein,Maltose-binding protein,MBP
2	B, C, D, E (B, C, D, E)	Maltose/maltodextrin-binding periplasmic protein,Polymerase cofactor VP35	polymer	671	73655.6	4	UniProt (P0AEX9) UniProt (P35259) Pfam (PF02097)	Escherichia coli K-12	MMBP,Maltodextrin-binding protein,Maltose-binding protein,MBP,Marburg VP35,mVP35
3	F (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)

Sequence modifications

A: 1 - 1425 (UniProt: P31352)

PDB	External Database	Details
Ala 489	Leu 489	conflict
Gly 979	Arg 979	conflict
Ser 1426	-	linker
Arg 1427	-	linker
Glu 1428	-	linker
Asn 1429	-	linker
Leu 1430	-	linker
Tyr 1431	-	linker
Phe 1432	-	linker
Gln 1433	-	linker
Gly 1434	-	linker
Ser 1435	-	linker
Gly 1436	-	linker
Trp 1437	-	linker
Ser 1438	-	linker
His 1439	-	linker
Pro 1440	-	linker
Gln 1441	-	linker
Phe 1442	-	linker
Glu 1443	-	linker
Lys 1444	-	linker
Gly 1445	-	linker
Gly 1446	-	linker
Gly 1447	-	linker
Ser 1448	-	linker
Gly 1449	-	linker
Gly 1450	-	linker
Gly 1451	-	linker
Ser 1452	-	linker
Gly 1453	-	linker
Gly 1454	-	linker
Ser 1455	-	linker
Ala 1456	-	linker
Trp 1457	-	linker
Ser 1458	-	linker
His 1459	-	linker
Pro 1460	-	linker
Gln 1461	-	linker
Phe 1462	-	linker
Glu 1463	-	linker
Lys 1464	-	linker
Gly 1465	-	linker
Ser 1466	-	linker
Ala 1467	-	linker
Ser 1468	-	linker
His 1469	-	linker
His 1470	-	linker
His 1471	-	linker
His 1472	-	linker
His 1473	-	linker
His 1474	-	linker
Gly 1475	-	linker
Thr 1476	-	linker
Lys 1477	-	linker
Thr 1478	-	linker

A: 1479 - 1842 (UniProt: P0AEX9)

PDB	External Database	Details
Gly 1843	-	expression tag
Asp 1844	-	expression tag
Tyr 1845	-	expression tag
Lys 1846	-	expression tag
Asp 1847	-	expression tag
Asp 1848	-	expression tag
Asp 1849	-	expression tag
Asp 1850	-	expression tag
Lys 1851	-	expression tag

B, C, D, E: -327 - 36 (UniProt: P0AEX9)

PDB	External Database	Details
Met -341	-	initiating methionine
Gly -340	-	expression tag
Ser -339	-	expression tag
Ser -338	-	expression tag
His -337	-	expression tag
His -336	-	expression tag
His -335	-	expression tag
His -334	-	expression tag
His -333	-	expression tag
His -332	-	expression tag
Gly -331	-	expression tag
Thr -330	-	expression tag
Lys -329	-	expression tag
Thr -328	-	expression tag
Gly 37	-	linker
Thr 38	-	linker
Asp 39	-	linker
Tyr 40	-	linker
Asp 41	-	linker
Ile 42	-	linker
Pro 43	-	linker
Thr 44	-	linker
Thr 45	-	linker
Leu 46	-	linker
Glu 47	-	linker
Val 48	-	linker
Leu 49	-	linker
Phe 50	-	linker
Gln 51	-	linker
Gly 52	-	linker
Pro 53	-	linker
Leu 54	-	linker
Gly 55	-	linker
Ser 56	-	linker

B, C, D, E: 57 - 329 (UniProt: P35259)

PDB	External Database	Details
Cys 296	Ser 296	conflict

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	504775.3
Non-Polymers*	Number of molecules	1
	Total formula weight	65.4
All*	Total formula weight	504840.7

*Water molecules are not included.