9IM4

Crystal Structure of AF9 YEATS domain F28R mutant in complex with histone H3K9la

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, B)	Protein AF-9	polymer	158	18510.3	2	UniProt (P42568) Pfam (PF03366)	Homo sapiens (human)	ALL1-fused gene from chromosome 9 protein,Myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein,YEATS domain-containing protein 3
2	B, D (P, C)	Histone H3.3C	polymer	10	1221.4	2	UniProt (Q6NXT2)	Homo sapiens	Histone H3.5
3	E (B)	water	water		18.0	1	Chemie (HOH)

Sequence modifications

A, B: 1 - 138 (UniProt: P42568)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Arg 28	Phe 28	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	39463.3
All*	Total formula weight	39463.3

*Water molecules are not included.