9IK9

Cryo-EM Structure of SST analogs bond SSTR1-Gi complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	350	40022.5	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	373	41276.3	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
3	C (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (E)	scFv16	polymer	338	36399.7	1		synthetic construct
5	E (D)	Somatostatin receptor type 1	polymer	393	42960.3	1	UniProt (P30872) Pfam (PF00001)	Homo sapiens (human)	SS-1-R,SS1-R,SS1R,SST1,SRIF-2
6	F (F)	(4J2)(DCY)(DTY)(DTR)K(DVA)(DCY)(ALO)(NH2)	polymer	9	1098.3	1		synthetic construct

Sequence modifications

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -21	-	initiating methionine
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag
Val 341	-	expression tag
Ser 342	-	expression tag
Gly 343	-	expression tag
Trp 344	-	expression tag
Arg 345	-	expression tag
Leu 346	-	expression tag
Phe 347	-	expression tag
Lys 348	-	expression tag
Lys 349	-	expression tag
Ile 350	-	expression tag
Ser 351	-	expression tag

D: 1 - 391 (UniProt: P30872)

PDB	External Database	Details
Leu 392	-	expression tag
Glu 393	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	169618.2
All*	Total formula weight	169618.2

*Water molecules are not included.