9IF7
Cryo-EM structure of the kinetoplastid trans-spliceosome activated for catalytic step II (C* complex)
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (L7) | Probable eukaryotic initiation factor 4A | polymer | 389 | 44002.8 | 1 | UniProt (A0A640KRB9) | Leishmania tarentolae | ATP-dependent RNA helicase eIF4A |
| 2 | B (L8) | RRM domain-containing protein | polymer | 153 | 17154.0 | 1 | UniProt (A0A640KP41) | Leishmania tarentolae | |
| 3 | C (L9) | Mago nashi-like protein,putative | polymer | 162 | 18684.2 | 1 | UniProt (A0A640KMM3) | Leishmania tarentolae | |
| 4 | D (LA) | Prp8 protein homologue, putative | polymer | 2427 | 278149.2 | 1 | UniProt (A0A640KTJ2) | Leishmania tarentolae | |
| 5 | E (LB) | ATP-dependent RNA helicase, putative | polymer | 2367 | 261270.3 | 1 | UniProt (A0A640KDJ4) | Leishmania tarentolae | |
| 6 | F (LC) | Small nuclear ribonucleoprotein component-like protein | polymer | 1016 | 110616.1 | 1 | UniProt (A0A640KRB5) | Leishmania tarentolae | |
| 7 | G (LD) | PRKR-interacting protein 1 | polymer | 166 | 18266.3 | 1 | UniProt (A0A640KD47) | Leishmania tarentolae | |
| 8 | H (LF) | Splicing factor Cactin C-terminal domain-containing protein | polymer | 706 | 78806.5 | 1 | UniProt (A0A640KRR3) | Leishmania tarentolae | |
| 9 | I (LG) | RNA-binding motif protein | polymer | 82 | 8723.9 | 1 | Leishmania tarentolae | ||
| 10 | J (LH) | Splicing regulator SDE2-like protein | polymer | 159 | 17569.5 | 1 | Leishmania tarentolae | ||
| 11 | K (LJ) | Guanine nucleotide-binding protein subunit beta-like protein | polymer | 509 | 55427.6 | 1 | UniProt (A0A640KWH7) | Leishmania tarentolae | |
| 12 | L (LL) | G10 protein | polymer | 326 | 35590.6 | 1 | UniProt (A0A640KB76) | Leishmania tarentolae | |
| 13 | M (LN) | Guanine nucleotide-binding protein subunit beta-like protein | polymer | 322 | 33586.7 | 1 | UniProt (A0A640KKK2) | Leishmania tarentolae | |
| 14 | N (LP) | Spliceosome-associated CWC15-like protein | polymer | 207 | 22580.8 | 1 | UniProt (A0A640KWX5) | Leishmania tarentolae | |
| 15 | O (LQ) | Cell division control protein | polymer | 804 | 89110.7 | 1 | UniProt (A0A640KDH5) | Leishmania tarentolae | |
| 16 | P (LR) | CWF21 domain-containing protein | polymer | 197 | 21499.0 | 1 | UniProt (A0A640K740) | Leishmania tarentolae | |
| 17 | Q (LS) | Crooked neck-like protein 1 | polymer | 824 | 93722.8 | 1 | UniProt (A0A640KV53) | Leishmania tarentolae | |
| 18 | R (LT) | Pre-mRNA-splicing factor SYF1 | polymer | 794 | 89299.9 | 1 | UniProt (A0A640KGK2) | Leishmania tarentolae | |
| 19 | S (LV) | RNA helicase | polymer | 1087 | 123210.9 | 1 | UniProt (A0A640KVV3) | Leishmania tarentolae | |
| 20 | T (LW) | U2 small nuclear ribonucleoprotein 40K, putative | polymer | 307 | 34821.8 | 1 | UniProt (A0A640KNR9) | Leishmania tarentolae | |
| 21 | U (LY) | RRM domain-containing protein | polymer | 114 | 13295.3 | 1 | UniProt (A0A640KKX2) | Leishmania tarentolae | |
| 22 | V (LZ) | NF-kappa-B-activating protein C-terminal domain-containing protein | polymer | 403 | 43596.1 | 1 | UniProt (A0A640KXH6) | Leishmania tarentolae | |
| 23 | W (SA) | MI domain-containing protein | polymer | 564 | 65686.4 | 1 | UniProt (A0A640KX85) | Leishmania tarentolae | |
| 24 | X (SC) | Pre-mRNA-splicing factor SLU7 | polymer | 417 | 46469.1 | 1 | UniProt (A0A640KB40) | Leishmania tarentolae | |
| 25 | Y (SD) | Pre-mRNA-splicing factor 18 | polymer | 546 | 59023.4 | 1 | UniProt (A0A640KFR7) | Leishmania tarentolae | |
| 26 | Z (SF) | Uncharacterized protein | polymer | 258 | 27783.3 | 1 | UniProt (A0A640KDU3) | Leishmania tarentolae | |
| 27 | AA (SJ) | PRP19-related complex protein 3 | polymer | 414 | 45326.1 | 1 | UniProt (A0A640KRN7) | Leishmania tarentolae | |
| 28 | BA (SK) | Unspecified product | polymer | 175 | 18754.7 | 1 | UniProt (A0A640KWM9) | Leishmania tarentolae | |
| 29 | CA (SL) | SNW domain-containing protein 1 | polymer | 544 | 57655.5 | 1 | Leishmania tarentolae | ||
| 30 | DA (SN) | peptidylprolyl isomerase | polymer | 192 | 20554.6 | 1 | UniProt (A0A640KHX2) | Leishmania tarentolae | |
| 31 | EA (SO) | Guanine nucleotide-binding protein subunit beta-like protein | polymer | 621 | 68007.3 | 1 | UniProt (A0A640KJ83) | Leishmania tarentolae | |
| 32 | FA (SP) | Pre-mRNA-splicing factor ISY1 homolog | polymer | 269 | 31012.2 | 1 | UniProt (A0A640KE26) | Leishmania tarentolae | |
| 33 | GA (SQ) | Zinc knuckle | polymer | 150 | 16757.0 | 1 | UniProt (A0A640KSY0) | Leishmania tarentolae | |
| 34 | HA (SS) | Pre-mRNA-splicing factor SPF27 | polymer | 284 | 31636.9 | 1 | UniProt (A0A640KPK5) | Leishmania tarentolae | |
| 35 | IA, JA, KA, LA (ST, SU, SV, SW) | Pre-mRNA-processing factor 19 | polymer | 513 | 53716.5 | 4 | UniProt (A0A640KLK2) | Leishmania tarentolae | |
| 36 | MA (TN) | Probable RNA helicase | polymer | 1181 | 128353.8 | 1 | Leishmania tarentolae | ||
| 37 | NA (UX) | Unidentified protein | polymer | 21 | 1805.2 | 1 | Leishmania tarentolae | ||
| 38 | OA (62) | LSM domain-containing protein | polymer | 190 | 20523.9 | 1 | UniProt (A0A640KDV9) | Leishmania tarentolae | |
| 39 | PA (63) | Sm domain-containing protein | polymer | 134 | 14153.9 | 1 | UniProt (A0A640K801) | Leishmania tarentolae | |
| 40 | QA (64) | U6 snRNA-associated Sm-like protein LSm4 | polymer | 116 | 12885.9 | 1 | UniProt (A0A640KQL9) | Leishmania tarentolae | |
| 41 | RA (65) | LSM domain-containing protein | polymer | 151 | 15939.7 | 1 | UniProt (A0A640KR67) | Leishmania tarentolae | |
| 42 | SA (66) | Sm domain-containing protein | polymer | 80 | 8616.6 | 1 | UniProt (A0A640K754) | Leishmania tarentolae | |
| 43 | TA (67) | Lsm7p protein, putative | polymer | 91 | 9968.6 | 1 | UniProt (A0A640KI01) | Leishmania tarentolae | |
| 44 | UA (68) | Sm domain-containing protein | polymer | 131 | 14106.0 | 1 | UniProt (A0A640KIZ1) | Leishmania tarentolae | |
| 45 | VA (RN) | Y-branched pre-mRNA | polymer | 68 | 13704.8 | 1 | Leishmania tarentolae | ||
| 46 | DB, PB, WA (24, 51, U1) | Small nuclear ribonucleoprotein, putative | polymer | 102 | 11214.8 | 3 | UniProt (A0A640KHR4) | Leishmania tarentolae | |
| 47 | EB, QB, XA (25, 52, U2) | Small nuclear ribonucleoprotein Sm D2 | polymer | 103 | 11798.7 | 3 | UniProt (A0A640KR16) | Leishmania tarentolae | Sm-D2,snRNP core protein D2 |
| 48 | YA (2C) | Lsm5p, putative | polymer | 128 | 13888.8 | 1 | UniProt (A0A640KNU1) | Leishmania tarentolae | |
| 49 | ZA (2D) | Small nuclear ribonucleaoprotein, putative | polymer | 141 | 16127.7 | 1 | UniProt (A0A640KZZ6) | Leishmania tarentolae | |
| 50 | AB, HB, TB (2H, 5E, UE) | Small nuclear ribonucleoprotein E | polymer | 86 | 9926.4 | 3 | UniProt (A0A640KLY5) | Leishmania tarentolae | snRNP-E,Sm protein E |
| 51 | BB, IB, UB (2I, 5F, UF) | Sm protein F | polymer | 76 | 8533.7 | 3 | UniProt (A0A640KUD7) | Leishmania tarentolae | |
| 52 | CB, JB, VB (2J, 5G, UG) | Sm protein G | polymer | 81 | 8810.2 | 3 | UniProt (A0A640KQ84) | Leishmania tarentolae | |
| 53 | FB, RB (53, U3) | Small nuclear ribonucleoprotein Sm D3 | polymer | 113 | 12345.2 | 2 | UniProt (A0A640KTK0) | Leishmania tarentolae | Sm-D3,snRNP core protein D3 |
| 54 | GB, SB (5B, UB) | Sm protein B | polymer | 111 | 11872.8 | 2 | UniProt (A0A640KQU2) | Leishmania tarentolae | |
| 55 | KB (LE) | SL RNA exon | polymer | 39 | 12361.3 | 1 | Leishmania tarentolae | ||
| 56 | LB (LO) | SL RNA outron | polymer | 57 | 18331.9 | 1 | GenBank (312489) | Leishmania tarentolae | |
| 57 | MB (N2) | U2 snRNA | polymer | 142 | 45393.7 | 1 | GenBank (15822519) | Leishmania tarentolae | |
| 58 | NB (N5) | U5 snRNA | polymer | 71 | 22591.4 | 1 | GenBank (2624234246) | Leishmania tarentolae | |
| 59 | OB (N6) | U6 snRNA | polymer | 100 | 32113.1 | 1 | GenBank (15824497) | Leishmania tarentolae | |
| 60 | AC, CC, JC, LC, MC... (LA, LC, N5, N6, L7) | MAGNESIUM ION | non-polymer | 24.3 | 12 | Chemie (MG) PubChem (888) | |||
| 61 | XB (L7) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 1 | Chemie (ADP) | |||
| 62 | YB (L7) | POTASSIUM ION | non-polymer | 39.1 | 1 | Chemie (K) PubChem (813) | |||
| 63 | ZB (LA) | INOSITOL HEXAKISPHOSPHATE | non-polymer | 660.0 | 1 | Chemie (IHP) | |||
| 64 | BC (LC) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) | |||
| 65 | DC, EC, FC, GC, HC (LL, SC, SQ) | ZINC ION | non-polymer | 65.4 | 5 | Chemie (ZN) PubChem (32051) | |||
| 66 | IC (N5) | 7-METHYL-GUANOSINE-5'-TRIPHOSPHATE-5'-GUANOSINE | non-polymer | 803.4 | 1 | Chemie (GTG) | |||
| 67 | KC (N6) | 5'-O-[(S)-hydroxy{[(R)-hydroxy{[(S)-hydroxy(methoxy)phosphoryl]oxy}phosphoryl]oxy}phosphoryl]guanosine | non-polymer | 537.2 | 1 | Chemie (G5J) |
Sequence modifications
SQ: 1 - 149 (UniProt: A0A640KSY0)
68: 1 - 130 (UniProt: A0A640KIZ1)
| PDB | External Database | Details |
|---|---|---|
| Ace 0 | - | acetylation |
| PDB | External Database | Details |
|---|---|---|
| Ace 0 | - | acetylation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 74 |
| Total formula weight | 2812671.5 | |
| Non-Polymers* | Number of molecules | 23 |
| Total formula weight | 3608.9 | |
| All* | Total formula weight | 2816280.4 |
