9I8V

Cryo-EM structure of the Danio rerio tRNA ligase complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	ATP-dependent RNA helicase	polymer	48	5349.1	1	UniProt (F1R2L8)	Danio rerio (zebrafish)
2	B (C)	RNA-splicing ligase RtcB homolog	polymer	519	57225.3	1	UniProt (Q6NZS4) Pfam (PF01139)	Danio rerio (zebrafish)	3'-phosphate/5'-hydroxy nucleic acid ligase
3	C (D)	Family with sequence similarity 98, member B	polymer	296	33196.7	1	UniProt (A0PJS3) Pfam (PF10239)	Danio rerio (zebrafish)	Protein FAM98B
4	D (E)	RNA transcription, translation and transport factor protein	polymer	242	27911.8	1	UniProt (Q7ZUH1) Pfam (PF10036)	Danio rerio (zebrafish)
5	E (F)	Ashwin	polymer	83	9706.1	1	UniProt (Q32LR5) Pfam (PF15323)	Danio rerio (zebrafish)

Sequence modifications

C: 1 - 505 (UniProt: Q6NZS4)

PDB	External Database	Details
Met -13	-	initiating methionine
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag

E: 1 - 242 (UniProt: Q7ZUH1)

PDB	External Database	Details
Ile 201	Val 201	variant

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	133389.0
All*	Total formula weight	133389.0

*Water molecules are not included.