9HS3
Crystal structure of the Escherichia coli nucleosidase PpnN (E264A, pppGpp form)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Pyrimidine/purine nucleotide 5'-monophosphate nucleosidase | polymer | 458 | 51412.3 | 4 | UniProt (P0ADR8) | Escherichia coli K-12 | AMP nucleosidase,CMP nucleosidase,GMP nucleosidase,IMP nucleosidase,UMP nucleosidase,dTMP nucleosidase |
| 2 | E, G, I, J (A, B, C, D) | 5-O-phosphono-alpha-D-ribofuranose | non-polymer | 230.1 | 4 | Chemie (HSX) | |||
| 3 | F, H, K, L (A, B, D) | guanosine 5'-(tetrahydrogen triphosphate) 3'-(trihydrogen diphosphate) | non-polymer | 683.1 | 4 | Chemie (0O2) | |||
| 4 | M, N, O, P (A, B, C, D) | water | water | 18.0 | 17 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 2 - 450 (UniProt: P0ADR8)
| PDB | External Database | Details |
|---|---|---|
| Pro -3 | - | expression tag |
| Ala -2 | - | expression tag |
| Leu -1 | - | expression tag |
| Arg 0 | - | expression tag |
| Met 1 | - | expression tag |
| Ala 264 | Glu 264 | engineered mutation |
| Glu 451 | - | expression tag |
| Ile 452 | - | expression tag |
| Cys 453 | - | expression tag |
| Thr 454 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 205649.0 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 3653.0 | |
| All* | Total formula weight | 209302.0 |
