9HS3
Crystal structure of the Escherichia coli nucleosidase PpnN (E264A, pppGpp form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-01 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 0.97623 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 156.919, 156.919, 224.654 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.420 - 3.400 |
| R-factor | 0.2211 |
| Rwork | 0.221 |
| R-free | 0.27790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.425 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.420 | 3.620 |
| High resolution limit [Å] | 3.400 | 3.500 |
| Rmeas | 0.180 | 1.110 |
| Number of reflections | 39210 | 3526 |
| <I/σ(I)> | 6.92 | |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 2 | 1.01 |
| CC(1/2) | 0.980 | 0.520 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 6 mg/ml 0.4 KNa tartrate |
