9HNW

USP1-UAF1 bound to Lys63-linked diubiquitin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Ubiquitin carboxyl-terminal hydrolase 1	polymer	813	91786.0	1	UniProt (O94782) Pfam (PF00443)	Homo sapiens (human)	Deubiquitinating enzyme 1,hUBP,Ubiquitin thioesterase 1,Ubiquitin-specific-processing protease 1
2	B (B)	WD repeat-containing protein 48	polymer	712	80130.7	1	UniProt (Q8TAF3) Pfam (PF00400) Pfam (PF11816)	Homo sapiens (human)	USP1-associated factor 1,WD repeat endosomal protein,p80
3	C (C)	Polyubiquitin-B	polymer	76	8604.9	1	UniProt (P0CG47) Pfam (PF00240) UniProt (by SIFTS) (P0CG48)	Homo sapiens
4	D (D)	Polyubiquitin-B	polymer	76	8547.8	1	UniProt (P0CG47) Pfam (PF00240) UniProt (by SIFTS) (P0CG48)	Homo sapiens
5	E (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)

Sequence modifications

A: 21 - 785 (UniProt: O94782)

PDB	External Database	Details
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
His 2	-	expression tag
His 3	-	expression tag
Asp 4	-	expression tag
Tyr 5	-	expression tag
Asp 6	-	expression tag
Ile 7	-	expression tag
Pro 8	-	expression tag
Thr 9	-	expression tag
Thr 10	-	expression tag
Glu 11	-	expression tag
Asn 12	-	expression tag
Leu 13	-	expression tag
Tyr 14	-	expression tag
Phe 15	-	expression tag
Gln 16	-	expression tag
Gly 17	-	expression tag
Ala 18	-	expression tag
Met 19	-	expression tag
Gly 20	-	expression tag
Ala 670	Gly 670	engineered mutation
Ala 671	Gly 671	engineered mutation
Glu 786	-	expression tag
Arg 787	-	expression tag
Pro 788	-	expression tag
Leu 789	-	expression tag
Ser 790	-	expression tag
Asn 791	-	expression tag
Leu 792	-	expression tag
Glu 793	-	expression tag
Pro 794	-	expression tag
Ala 795	-	expression tag
Val 796	-	expression tag
Ser 797	-	expression tag
Arg 798	-	expression tag
His 799	-	expression tag
Ala 800	-	expression tag
Val 801	-	expression tag
Pro 802	-	expression tag
Ser 803	-	expression tag
Leu 804	-	expression tag
Ser 805	-	expression tag
Arg 806	-	expression tag
Ser 807	-	expression tag
Thr 808	-	expression tag
Arg 809	-	expression tag
Gly 810	-	expression tag

B: 1 - 672 (UniProt: Q8TAF3)

PDB	External Database	Details
Trp -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
Pro -15	-	expression tag
Gln -14	-	expression tag
Phe -13	-	expression tag
Glu -12	-	expression tag
Lys -11	-	expression tag
Gly -10	-	expression tag
Ala -9	-	expression tag
Leu -8	-	expression tag
Glu -7	-	expression tag
Val -6	-	expression tag
Leu -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
Pro -1	-	expression tag
Gly 0	-	expression tag
Leu 673	-	expression tag
Arg 674	-	expression tag
Ser 675	-	expression tag
Pro 676	-	expression tag
Arg 677	-	expression tag
Asn 678	-	expression tag
Ser 679	-	expression tag
Arg 680	-	expression tag
His 681	-	expression tag
Ala 682	-	expression tag
Val 683	-	expression tag
Pro 684	-	expression tag
Ser 685	-	expression tag
Leu 686	-	expression tag
Ser 687	-	expression tag
Arg 688	-	expression tag
Ser 689	-	expression tag
Thr 690	-	expression tag
Arg 691	-	expression tag
Gly 692	-	expression tag
Ser 693	-	expression tag

C: 1 - 75 (UniProt: P0CG47)

PDB	External Database	Details
Abu 76	-	linker

D: 1 - 76 (UniProt: P0CG47)

PDB	External Database	Details
Dab 63	Lys 63	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	189069.4
Non-Polymers*	Number of molecules	1
	Total formula weight	65.4
All*	Total formula weight	189134.8

*Water molecules are not included.