9HMA

Cryo-EM structure of apo human separase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Separin	polymer	2217	243151.1	1	UniProt (Q14674) Pfam (PF03568)	Homo sapiens (human)	Caspase-like protein ESPL1,Extra spindle poles-like 1 protein,Separase
2	B (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)

Sequence modifications

A: 1 - 2177 (UniProt: Q14674)

PDB	External Database	Details
Met -56	-	initiating methionine
Ser -55	-	expression tag
Gly -54	-	expression tag
Asp -53	-	expression tag
Tyr -52	-	expression tag
Lys -51	-	expression tag
Asp -50	-	expression tag
His -49	-	expression tag
Asp -48	-	expression tag
Gly -47	-	expression tag
Asp -46	-	expression tag
Tyr -45	-	expression tag
Lys -44	-	expression tag
Asp -43	-	expression tag
His -42	-	expression tag
Asp -41	-	expression tag
Ile -40	-	expression tag
Asp -39	-	expression tag
Tyr -38	-	expression tag
Lys -37	-	expression tag
Asp -36	-	expression tag
Asp -35	-	expression tag
Asp -34	-	expression tag
Asp -33	-	expression tag
Lys -32	-	expression tag
Ser -31	-	expression tag
Gly -30	-	expression tag
Pro -29	-	expression tag
Gly -28	-	expression tag
Gly -27	-	expression tag
Ser -26	-	expression tag
Gly -25	-	expression tag
Gly -24	-	expression tag
Ser -23	-	expression tag
Gly -22	-	expression tag
Gly -21	-	expression tag
Gly -20	-	expression tag
Ser -19	-	expression tag
Gly -18	-	expression tag
Gly -17	-	expression tag
Gly -16	-	expression tag
Ser -15	-	expression tag
Gly -14	-	expression tag
Glu -13	-	expression tag
Asn -12	-	expression tag
Leu -11	-	expression tag
Tyr -10	-	expression tag
Phe -9	-	expression tag
Gln -8	-	expression tag
Gly -7	-	expression tag
Gly -6	-	expression tag
Gly -5	-	expression tag
Ser -4	-	expression tag
Gly -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag
Asp 25	Ala 25	conflict
Val 116	Ala 116	conflict
Ser 1386	Arg 1329	conflict
Gln 1618	Arg 1561	conflict
His 2094	Arg 2037	conflict
Ser 2178	-	expression tag
Ser 2179	-	expression tag
Leu 2180	-	expression tag
Ala 2181	-	expression tag
Glu 2182	-	expression tag
Glu 2183	-	expression tag
Asn 2184	-	expression tag
Leu 2185	-	expression tag
Tyr 2186	-	expression tag
Phe 2187	-	expression tag
Gln 2188	-	expression tag
Ser 2189	-	expression tag
Trp 2190	-	expression tag
Ser 2191	-	expression tag
His 2192	-	expression tag
Pro 2193	-	expression tag
Gln 2194	-	expression tag
Phe 2195	-	expression tag
Glu 2196	-	expression tag
Lys 2197	-	expression tag
Gly 2198	-	expression tag
Gly 2199	-	expression tag
Gly 2200	-	expression tag
Ser 2201	-	expression tag
Gly 2202	-	expression tag
Gly 2203	-	expression tag
Gly 2204	-	expression tag
Ser 2205	-	expression tag
Gly 2206	-	expression tag
Gly 2207	-	expression tag
Gly 2208	-	expression tag
Ser 2209	-	expression tag
Trp 2210	-	expression tag
Ser 2211	-	expression tag
His 2212	-	expression tag
Pro 2213	-	expression tag
Gln 2214	-	expression tag
Phe 2215	-	expression tag
Glu 2216	-	expression tag
Lys 2217	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	243151.1
Non-Polymers*	Number of molecules	1
	Total formula weight	65.4
All*	Total formula weight	243216.5

*Water molecules are not included.