9HDR

Human LINE-1 ORF2p target-primed reverse transcription complex with EN domain resolved

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	LINE-1 retrotransposable element ORF2 protein	polymer	1797	206717.6	1	UniProt (P0AEX9) UniProt (Q12306) UniProt (O00370) Pfam (PF03372) Pfam (PF00078)	Escherichia coli (strain K12)	MMBP,Maltodextrin-binding protein,Maltose-binding protein,MBP,ORF2p
2	B (B)	Target DNA strand 1	polymer	37	11424.3	1		Homo sapiens
3	C (C)	Target DNA strand 2	polymer	30	9145.0	1		Homo sapiens
4	D (D)	Template P(A)30 RNA	polymer	30	9831.2	1		Homo sapiens
5	E (E)	Target DNA strand 3	polymer	26	7900.1	1		Homo sapiens
6	F (F)	Target DNA strand 4	polymer	19	5906.0	1		Homo sapiens
7	G (G)	Unassigned Nucleic Acid	polymer	4	1207.9	1		Homo sapiens
8	H (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
9	I (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
10	J (A)	2',3'-DIDEOXY-THYMIDINE-5'-TRIPHOSPHATE	non-polymer		466.2	1	Chemie (D3T)

Sequence modifications

A: -476 - -113 (UniProt: P0AEX9)

PDB	External Database	Details
Met -521	-	initiating methionine
Val -520	-	expression tag
His -519	-	expression tag
His -518	-	expression tag
His -517	-	expression tag
His -516	-	expression tag
His -515	-	expression tag
His -514	-	expression tag
His -513	-	expression tag
His -512	-	expression tag
Gly -511	-	expression tag
Gly -510	-	expression tag
Ser -509	-	expression tag
Ala -508	-	expression tag
Trp -507	-	expression tag
Ser -506	-	expression tag
His -505	-	expression tag
Pro -504	-	expression tag
Gln -503	-	expression tag
Phe -502	-	expression tag
Glu -501	-	expression tag
Lys -500	-	expression tag
Gly -499	-	expression tag
Gly -498	-	expression tag
Gly -497	-	expression tag
Ser -496	-	expression tag
Gly -495	-	expression tag
Gly -494	-	expression tag
Gly -493	-	expression tag
Ser -492	-	expression tag
Gly -491	-	expression tag
Gly -490	-	expression tag
Ser -489	-	expression tag
Ala -488	-	expression tag
Trp -487	-	expression tag
Ser -486	-	expression tag
His -485	-	expression tag
Pro -484	-	expression tag
Gln -483	-	expression tag
Phe -482	-	expression tag
Glu -481	-	expression tag
Lys -480	-	expression tag
Gly -479	-	expression tag
Gly -478	-	expression tag
Ser -477	-	expression tag
Val -167	Ala 338	conflict
Asn -112	-	linker
Ser -111	-	linker
Ser -110	-	linker
Ser -109	-	linker
Asn -108	-	linker
Asn -107	-	linker
Asn -106	-	linker
Asn -105	-	linker
Asn -104	-	linker
Asn -103	-	linker
Asn -102	-	linker
Asn -101	-	linker
Asn -100	-	linker
Asn -99	-	linker
Leu -98	-	linker
Gly -97	-	linker

A: -96 - 0 (UniProt: Q12306)

PDB	External Database	Details
Thr -34	Arg 64	conflict
Glu -27	Arg 71	conflict

A: 1 - 1275 (UniProt: O00370)

PDB	External Database	Details
Thr 5	Asn 5	conflict
Ile 15	Val 15	conflict
Ala 21	Pro 21	conflict
Thr 152	Ile 152	conflict
Ala 175	Thr 175	conflict
Met 595	Val 595	conflict
Thr 625	Met 625	conflict
Phe 627	Leu 627	conflict
Thr 744	Asn 744	conflict
Glu 795	Asp 795	conflict
Phe 932	Leu 932	conflict
Leu 1141	Val 1141	conflict
Asn 1182	Lys 1182	conflict
Glu 1183	Asp 1183	conflict
Lys 1212	Asn 1212	conflict

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	252132.1
Non-Polymers*	Number of molecules	3
	Total formula weight	555.9
All*	Total formula weight	252688.0

*Water molecules are not included.