9HDE

Crystal structure of methionine gamma-lyase (K209Q variant) from Brevibacterium aurantiacum in complex with PLP and alpha-ketobutyrate

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	Cystathionine gamma-synthase	polymer	413	42819.4	24	UniProt (A0A2H1K3G9)	Brevibacterium aurantiacum	Methionine gamma-lyase (MGL)
2	DB, EA, HA, QB, RA... (N, B, X, H, A)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	7	Chemie (PEG)
3	AA, AB, BA, CB, EB... (A, M, N, O, B...)	SULFATE ION	non-polymer		96.1	15	Chemie (SO4)
4	BB, CA, DA, FB, GB... (N, A, B, O, P...)	(2E)-2-{[(1E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}but-2-enoic acid	non-polymer		330.2	24	Chemie (4LM)
5	AC, BC, CC, DC, EC... (I, J, K, L, M...)	water	water		18.0	430	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X: 1 - 393 (UniProt: A0A2H1K3G9)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Gln 209	Lys 222	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	24
	Total formula weight	1027666.3
Non-Polymers*	Number of molecules	46
	Total formula weight	10109.3
All*	Total formula weight	1037775.6

*Water molecules are not included.