9HC0

Dark structure of the human metabotropic glutamate receptor 5 transmembrane domain bound to photoswitchable ligand alloswitch-1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Metabotropic glutamate receptor 5,Endolysin	polymer	444	49916.6	1	UniProt (P41594) UniProt (P00720)	Homo sapiens (human)	mGluR5,Lysis protein,Lysozyme,Muramidase
2	B, C, D, E, F (A)	OLEIC ACID	non-polymer		282.5	5	Chemie (OLA)
3	G (A)	2-chloranyl-~{N}-[2-methoxy-4-[(~{E})-pyridin-2-yldiazenyl]phenyl]benzamide	non-polymer		366.8	1	Chemie (4YI)
4	H (A)	water	water		18.0	22	Chemie (HOH)

Sequence modifications

A: 569 - 678 (UniProt: P41594)

PDB	External Database	Details
Ala 566	-	expression tag
Ala 567	-	expression tag
Ser 568	-	expression tag
Ala 579	Glu 579	engineered mutation
Tyr 667	Asn 667	engineered mutation
Ala 669	Ile 669	engineered mutation
Met 675	Gly 675	engineered mutation

A: 1002 - 679 (UniProt: P00720)

PDB	External Database	Details
Gly 1012	Arg 12	engineered mutation
Thr 1054	Cys 54	engineered mutation
Ala 1097	Cys 97	engineered mutation
Arg 1137	Ile 137	engineered mutation

A: 680 - 836 (UniProt: P41594)

PDB	External Database	Details
Ala 742	Thr 742	engineered mutation
Ala 753	Ser 753	engineered mutation
Ala 837	-	expression tag
Ala 838	-	expression tag
Ala 839	-	expression tag
His 840	-	expression tag
His 841	-	expression tag
His 842	-	expression tag
His 843	-	expression tag
His 844	-	expression tag
His 845	-	expression tag
His 846	-	expression tag
His 847	-	expression tag
His 848	-	expression tag
His 849	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	49916.6
Non-Polymers*	Number of molecules	6
	Total formula weight	1779.1
All*	Total formula weight	51695.7

*Water molecules are not included.