9HC0
Dark structure of the human metabotropic glutamate receptor 5 transmembrane domain bound to photoswitchable ligand alloswitch-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-04-03 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 143.221, 43.363, 82.642 |
| Unit cell angles | 90.00, 99.86, 90.00 |
Refinement procedure
| Resolution | 49.310 - 2.330 |
| R-factor | 0.2332 |
| Rwork | 0.229 |
| R-free | 0.27110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.595 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20_4459) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.310 | 2.580 |
| High resolution limit [Å] | 2.330 | 2.330 |
| Rmerge | 0.082 | 0.907 |
| Number of reflections | 14590 | 128 |
| <I/σ(I)> | 8.8 | 1.4 |
| Completeness [%] | 67.1 | |
| Redundancy | 3.3 | |
| CC(1/2) | 0.997 | 0.618 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 7.2 | 293 | PEG300, di-ammonium hydrogen phosphate, 1,6-hexanediol, BIS-TRIS |
