9H9E

Cryo-EM structure of the human GABAA receptor alpha1 subunit in complex with the assembly factor NACHO/TMEM35A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, D)	Gamma-aminobutyric acid receptor subunit alpha-1	polymer	484	54100.1	2	UniProt (P14867) Pfam (PF02931) Pfam (PF02932)	Homo sapiens (human)	GABA(A) receptor subunit alpha-1
2	B, C (B, C)	Novel acetylcholine receptor chaperone	polymer	187	20141.9	2	UniProt (Q53FP2)	Homo sapiens (human)
3	E, F (a, d)	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-3)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		910.8	2	In PDB
4	G, K (A, D)	1,2-DIPALMITOYL-SN-GLYCERO-3-PHOSPHATE	non-polymer		647.9	2	Chemie (PX6)
5	H, L (A, D)	(7S)-4-HYDROXY-N,N,N-TRIMETHYL-9-OXO-7-[(PALMITOYLOXY)METHYL]-3,5,8-TRIOXA-4-PHOSPHAHEXACOSAN-1-AMINIUM 4-OXIDE	non-polymer		763.1	2	Chemie (PC7)
6	I, M (A, D)	(1S)-2-{[{[(2R)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL STEARATE	non-polymer		751.0	2	Chemie (PGT)
7	J, N (A, D)	CHOLESTEROL	non-polymer		386.7	2	Chemie (CLR)

Sequence modifications

A, D: 10 - 429 (UniProt: P14867)

PDB	External Database	Details
Met -54	-	initiating methionine
Gly -53	-	expression tag
Ile -52	-	expression tag
Leu -51	-	expression tag
Pro -50	-	expression tag
Ser -49	-	expression tag
Pro -48	-	expression tag
Gly -47	-	expression tag
Met -46	-	expression tag
Pro -45	-	expression tag
Ala -44	-	expression tag
Leu -43	-	expression tag
Leu -42	-	expression tag
Ser -41	-	expression tag
Leu -40	-	expression tag
Val -39	-	expression tag
Ser -38	-	expression tag
Leu -37	-	expression tag
Leu -36	-	expression tag
Ser -35	-	expression tag
Val -34	-	expression tag
Leu -33	-	expression tag
Leu -32	-	expression tag
Met -31	-	expression tag
Gly -30	-	expression tag
Cys -29	-	expression tag
Val -28	-	expression tag
Ala -27	-	expression tag
Glu -26	-	expression tag
Thr -25	-	expression tag
Gly -24	-	expression tag
Trp -23	-	expression tag
Ser -22	-	expression tag
His -21	-	expression tag
Pro -20	-	expression tag
Gln -19	-	expression tag
Phe -18	-	expression tag
Glu -17	-	expression tag
Lys -16	-	expression tag
Gly -15	-	expression tag
Gly -14	-	expression tag
Gly -13	-	expression tag
Ser -12	-	expression tag
Gly -11	-	expression tag
Gly -10	-	expression tag
Gly -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Gly -6	-	expression tag
Ser -5	-	expression tag
Ala -4	-	expression tag
Trp -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Pro 0	-	expression tag
Gln 1	-	expression tag
Phe 2	-	expression tag
Glu 3	-	expression tag
Lys 4	-	expression tag
Gly 5	-	expression tag
Gly 6	-	expression tag
Ser 7	-	expression tag
Thr 8	-	expression tag
Gly 9	-	expression tag

B, C: 1 - 167 (UniProt: Q53FP2)

PDB	External Database	Details
Gly 168	-	expression tag
Gly 169	-	expression tag
Ser 170	-	expression tag
Gly 171	-	expression tag
Gly 172	-	expression tag
Ser 173	-	expression tag
Gly 174	-	expression tag
Gly 175	-	expression tag
Ser 176	-	expression tag
Gly 177	-	expression tag
Lys 178	-	expression tag
Thr 179	-	expression tag
Glu 180	-	expression tag
Thr 181	-	expression tag
Ser 182	-	expression tag
Gln 183	-	expression tag
Val 184	-	expression tag
Ala 185	-	expression tag
Pro 186	-	expression tag
Ala 187	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	148484.0
Branched	Number of molecules	2
	Total formula weight	1821.6
Non-Polymers*	Number of molecules	8
	Total formula weight	5097.3
All*	Total formula weight	155403.0

*Water molecules are not included.