9H6C

Crystal structure of the E. coli F-plasmid VapBC toxin-antitoxin complex (VapB T3N)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	tRNA(fMet)-specific endonuclease VapC	polymer	132	14818.1	2	UniProt (Q84A22) Pfam (PF01850)	Escherichia coli KLY	RNase VapC,Toxin VapC
2	B, D (B, D)	Antitoxin	polymer	96	10947.1	2	UniProt (Q7B3V0) UniProt (by SIFTS) (P18355)	Escherichia coli KLY	VapB protein (Antitoxin to VapC)

Sequence modifications

B, D: 1 - 75 (UniProt: Q7B3V0)

PDB	External Database	Details
Met -20	-	initiating methionine
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
His 0	-	expression tag
Asn 3	Thr 3	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	51530.3
All*	Total formula weight	51530.3

*Water molecules are not included.