9H6C

Crystal structure of the E. coli F-plasmid VapBC toxin-antitoxin complex (VapB T3N)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979261
Spacegroup name	P 32 2 1
Unit cell lengths	88.974, 88.974, 115.153
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.490 - 2.650
R-factor	0.2334
Rwork	0.231
R-free	0.28420
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.931
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.500	44.500	2.570
High resolution limit [Å]	2.500	11.190	2.500
Rmerge	0.093	0.026	5.796
Rmeas	0.098	0.028	6.104
Number of reflections	18721	243	1376
<I/σ(I)>	13.21
Completeness [%]	99.9
Redundancy	10.07
CC(1/2)	0.999	0.999	0.215

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	0.2 M Lithium sulphate monohydrate, 0.1 M sodium citrate tribasic dihydrate, 26 % PEG200