9H59

Cryo-EM structure of DDB1-CRBN in complex with NK7-902 and NEK7

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA damage-binding protein 1	polymer	1140	127097.5	1	UniProt (Q16531) Pfam (PF10433) Pfam (PF23726) Pfam (PF03178)	Homo sapiens (human)	DDB p127 subunit,DNA damage-binding protein a,DDBa,Damage-specific DNA-binding protein 1,HBV X-associated protein 1,XAP-1,UV-damaged DNA-binding factor,UV-damaged DNA-binding protein 1,UV-DDB 1,XPE-binding factor,XPE-BF,Xeroderma pigmentosum group E-complementing protein,XPCe
2	B (C)	Serine/threonine-protein kinase Nek7	polymer	304	34753.1	1	UniProt (Q8TDX7) Pfam (PF00069)	Homo sapiens (human)	Never in mitosis A-related kinase 7,NimA-related protein kinase 7
3	C (B)	Protein cereblon	polymer	459	52517.8	1	UniProt (Q96SW2) Pfam (PF02190) Pfam (PF03226)	Homo sapiens (human)
4	D (B)	2-[(3S)-2,6-bis(oxidanylidene)piperidin-3-yl]-5-[(1S,2R,5S)-2-(ethylamino)-8-azabicyclo[3.2.1]octan-8-yl]isoindole-1,3-dione	non-polymer		410.5	1	Chemie (A1ISP)

Sequence modifications

C: 1 - 302 (UniProt: Q8TDX7)

PDB	External Database	Details
Gly -1	-	expression tag
Pro 0	-	expression tag

B: 2 - 442 (UniProt: Q96SW2)

PDB	External Database	Details
Met -16	-	initiating methionine
Gly -15	-	expression tag
Gly -14	-	expression tag
Ser -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
Leu -6	-	expression tag
Glu -5	-	expression tag
Val -4	-	expression tag
Leu -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag
Pro 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	214368.4
Non-Polymers*	Number of molecules	1
	Total formula weight	410.5
All*	Total formula weight	214778.8

*Water molecules are not included.