9H39

Crystal structure of Lotus japonicus CHIP13 extracellular domain in complex with a nanobody

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	LysM type receptor kinase	polymer	227	25008.7	2	UniProt (D3KU00) Pfam (PF01476)	Lotus japonicus
2	C, D (C, D)	anti-LYS13 VHH	polymer	128	14507.1	2		Lama glama
3	E (N)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	1	In PDB GlyTouCan (G15407YE)
4	F, G (E, M)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	2	In PDB GlyTouCan (G42666HT)
5	H (F)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		732.7	1	In PDB GlyTouCan (G32152BH)
6	I (G)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		570.5	1	In PDB GlyTouCan (G21290RB)
7	J, K (B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)
8	L, M, N, O (A, B, C, D)	water	water		18.0	582	Chemie (HOH)

Sequence modifications

A, B: 33 - 253 (UniProt: D3KU00)

PDB	External Database	Details
His 254	-	expression tag
His 255	-	expression tag
His 256	-	expression tag
His 257	-	expression tag
His 258	-	expression tag
His 259	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	79031.6
Branched	Number of molecules	5
	Total formula weight	2738.6
Non-Polymers*	Number of molecules	2
	Total formula weight	442.4
All*	Total formula weight	82212.6

*Water molecules are not included.