9H39

Crystal structure of Lotus japonicus CHIP13 extracellular domain in complex with a nanobody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-02-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97
Spacegroup name	P 1 21 1
Unit cell lengths	68.900, 55.090, 109.340
Unit cell angles	90.00, 98.91, 90.00

Refinement procedure

Resolution	49.080 - 1.660
R-factor	0.1835
Rwork	0.182
R-free	0.22510
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.677
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.080	1.690
High resolution limit [Å]	1.660	1.660
Rmerge	0.056	3.452
Rmeas	0.061	3.726
Rpim	0.024	1.391
Number of reflections	94949	4915
<I/σ(I)>	11.74
Completeness [%]	98.5
Redundancy	6.9
CC(1/2)	0.997	0.364

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	292.15	0.2 M sodium acetate 0.1 M Bis Tris propane 7.5 20 % w/v PEG 3350