9GP7

Cryo EM structure of the E307T mutant of the human P2X4 receptor in complex with the anthraquinone derivative PSB-0704

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	P2X purinoceptor 4	polymer	412	46303.2	3	UniProt (Q99571) Pfam (PF00864)	Homo sapiens (human)	P2X4,ATP receptor,Purinergic receptor
2	D, E, F (D, E, F)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	3	In PDB GlyTouCan (G42666HT)
3	G, I, K (A, C)	1-azanyl-4-[(3-carboxy-4-phenylazanyl-phenyl)amino]-9,10-bis(oxidanylidene)anthracene-2-carboxylic acid	non-polymer		493.5	3	Chemie (A1INJ)
4	H, J, L (A, B, C)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	3	Chemie (NAG)

Sequence modifications

A, B, C: 1 - 388 (UniProt: Q99571)

PDB	External Database	Details
Arg 75	Asn 75	engineered mutation
Arg 184	Asn 184	engineered mutation
Thr 307	Glu 307	engineered mutation
Gly 389	-	expression tag
Ser 390	-	expression tag
Ser 391	-	expression tag
Gly 392	-	expression tag
Glu 393	-	expression tag
Phe 394	-	expression tag
Leu 395	-	expression tag
Glu 396	-	expression tag
Val 397	-	expression tag
Leu 398	-	expression tag
Phe 399	-	expression tag
Gln 400	-	expression tag
Gly 401	-	expression tag
Pro 402	-	expression tag
His 403	-	expression tag
His 404	-	expression tag
His 405	-	expression tag
His 406	-	expression tag
His 407	-	expression tag
His 408	-	expression tag
His 409	-	expression tag
His 410	-	expression tag
His 411	-	expression tag
His 412	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	138909.6
Branched	Number of molecules	3
	Total formula weight	1273.2
Non-Polymers*	Number of molecules	6
	Total formula weight	2144.0
All*	Total formula weight	142326.8

*Water molecules are not included.