9GNK
Desulfovibrio desulfuricans [FeFe]-hydrogenase variant with both subunits linked by a 13 amino acid linker peptide derived from CpI of Clostridium pasteurianum
Replaces: 8RU7Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Periplasmic [Fe] hydrogenase large subunit,Periplasmic [Fe] hydrogenase small subunit | polymer | 492 | 54312.2 | 1 | UniProt (P07598) UniProt (P07603) Pfam (PF00037) Pfam (PF02906) Pfam (PF02256) | Desulfovibrio desulfuricans | Fe hydrogenlyase,Fe hydrogenlyase small chain |
| 2 | B (A) | CARBON MONOXIDE | non-polymer | 28.0 | 1 | Chemie (CMO) | |||
| 3 | C (A) | dicarbonyl[bis(cyanide-kappaC)]-mu-(iminodimethanethiolatato-1kappaS:2kappaS)-mu-(oxomethylidene)diiron(2+) | non-polymer | 355.0 | 1 | Chemie (402) | |||
| 4 | D, E, F (A) | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 3 | Chemie (SF4) | |||
| 5 | G (A) | 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID | non-polymer | 238.3 | 1 | Chemie (EPE) | |||
| 6 | H, L, M (A) | TETRAETHYLENE GLYCOL | non-polymer | 194.2 | 3 | Chemie (PG4) | |||
| 7 | I, J, K (A) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 3 | Chemie (PEG) | |||
| 8 | N (A) | water | water | 18.0 | 543 | Chemie (HOH) |
Sequence modifications
A: 1 - 390 (UniProt: P07598)
A: 404 - 482 (UniProt: P07603)
| PDB | External Database | Details |
|---|---|---|
| Val 391 | - | linker |
| Asn 392 | - | linker |
| Pro 393 | - | linker |
| Lys 394 | - | linker |
| Asp 395 | - | linker |
| Leu 396 | - | linker |
| Glu 397 | - | linker |
| Lys 398 | - | linker |
| Val 399 | - | linker |
| Asp 400 | - | linker |
| Ile 401 | - | linker |
| Lys 402 | - | linker |
| Lys 403 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Ser 483 | - | expression tag |
| Ala 484 | - | expression tag |
| Trp 485 | - | expression tag |
| Ser 486 | - | expression tag |
| His 487 | - | expression tag |
| Pro 488 | - | expression tag |
| Gln 489 | - | expression tag |
| Phe 490 | - | expression tag |
| Glu 491 | - | expression tag |
| Lys 492 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 54312.2 | |
| Non-Polymers* | Number of molecules | 12 |
| Total formula weight | 2577.2 | |
| All* | Total formula weight | 56889.4 |
