9GGC

Structure of the G848S mutant of human mitochondrial DNA polymerase gamma

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA polymerase subunit gamma-1	polymer	1221	138074.7	1	UniProt (P54098)	Homo sapiens (human)	3'-5' exodeoxyribonuclease,5'-deoxyribose-phosphate lyase,Mitochondrial DNA polymerase catalytic subunit,PolG-alpha
2	B, C (B, C)	DNA polymerase subunit gamma-2	polymer	467	53229.7	2	UniProt (Q9UHN1)	Homo sapiens (human)	DNA polymerase gamma accessory 55 kDa subunit,p55,Mitochondrial DNA polymerase accessory subunit,MtPolB,PolG-beta
3	D (P)	DNA (primer strand)	polymer	25	7765.0	1		synthetic construct
4	E (T)	DNA (template strand)	polymer	40	12162.8	1		synthetic construct
5	F (A)	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
6	G (A)	2'-DEOXYCYTIDINE-5'-TRIPHOSPHATE	non-polymer		467.2	1	Chemie (DCP)

Sequence modifications

A: 26 - 1239 (UniProt: P54098)

PDB	External Database	Details
Met 19	-	initiating methionine
His 20	-	expression tag
His 21	-	expression tag
His 22	-	expression tag
His 23	-	expression tag
His 24	-	expression tag
His 25	-	expression tag
Ser 848	Gly 848	engineered mutation

B, C: 26 - 485 (UniProt: Q9UHN1)

PDB	External Database	Details
Met 25	-	initiating methionine
Thr 169	Ala 169	variant
His 486	-	expression tag
His 487	-	expression tag
His 488	-	expression tag
His 489	-	expression tag
His 490	-	expression tag
His 491	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	264461.8
Non-Polymers*	Number of molecules	2
	Total formula weight	507.2
All*	Total formula weight	264969.1

*Water molecules are not included.