9GEK

Structure of the FAST1-FAST2-RAP module from human FASTKD4 by carrier-driven crystallisation with maltose binding protein from E. coli.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Maltose/maltodextrin-binding periplasmic protein,FAST kinase domain-containing protein 4,FAST kinase domain-containing protein 4	polymer	725	80110.3	1	UniProt (Q969Z0) Pfam (PF06743) Pfam (PF08368) Pfam (PF08373)	Homo sapiens (human)	Cell cycle progression restoration protein 2,Cell cycle progression protein 2,Protein TBRG4,Transforming growth factor beta regulator 4
2	B (A)	3,6,9,12,15,18-HEXAOXAICOSANE-1,20-DIOL	non-polymer		326.4	1	Chemie (P33)
3	C (A)	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)
4	D, F (A)	alpha-D-glucopyranose	non-polymer		180.2	2	Chemie (GLC)
5	E, G (A)	beta-D-fructofuranose	non-polymer		180.2	2	Chemie (FRU)
6	H (A)	water	water		18.0	261	Chemie (HOH)

Sequence modifications

A: -85 - 306 (PDB: 9GEK)

PDB	External Database	Details
Gly 307	-	linker
Thr 308	-	linker

A: 309 - 631 (UniProt: Q969Z0)

PDB	External Database	Details
Asp 487	Arg 487	engineered mutation
Leu 632	-	expression tag
Glu 633	-	expression tag
His 634	-	expression tag
His 635	-	expression tag
His 636	-	expression tag
His 637	-	expression tag
His 638	-	expression tag
His 639	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	80110.3
Non-Polymers*	Number of molecules	6
	Total formula weight	1142.0
All*	Total formula weight	81252.3

*Water molecules are not included.