9GEK
Structure of the FAST1-FAST2-RAP module from human FASTKD4 by carrier-driven crystallisation with maltose binding protein from E. coli.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-02 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.000027 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 80.058, 88.315, 114.809 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.130 - 2.150 |
| R-factor | 0.2044 |
| Rwork | 0.203 |
| R-free | 0.24070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.552 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21.1_5286: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.130 | 2.280 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmeas | 0.190 | 2.150 |
| Number of reflections | 85063 | 14074 |
| <I/σ(I)> | 10.9 | 1.1 |
| Completeness [%] | 1.0 | 0.982 |
| Redundancy | 11.2 | 9 |
| CC(1/2) | 1.000 | 0.360 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 298 | 15% (v/v) PEG 8.000, 60 mM potassium phosphate |
