9GDR

Cryo-EM structure of Vibrio cholerae RNA polymerase Transcription Activation Complex with TcpP transcription factor and a toxT promoter DNA fragment

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (H, K)	Toxin co-regulated pilus biosynthesis protein P	polymer	115	13447.5	2	UniProt (A0A0H3AHV1)	Vibrio cholerae O395
2	C (N)	toxT promoter non-template DNA strand	polymer	71	21952.1	1		Vibrio cholerae O395
3	D (T)	toxT promoter template DNA strand	polymer	71	21918.2	1		Vibrio cholerae O395
4	E, I, J (G, A, B)	DNA-directed RNA polymerase subunit alpha	polymer	330	36461.5	3	UniProt (A5F572)	Vibrio cholerae O395	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
5	F (C)	DNA-directed RNA polymerase subunit beta	polymer	1341	149696.5	1	UniProt (A5F3P5)	Vibrio cholerae O395	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
6	G (D)	DNA-directed RNA polymerase subunit beta'	polymer	1401	155234.3	1	UniProt (A5F3P6)	Vibrio cholerae O395	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
7	H (E)	DNA-directed RNA polymerase subunit omega	polymer	90	10068.4	1	UniProt (A5F4S8)	Vibrio cholerae O395	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
8	K (F)	RNA polymerase sigma factor RpoD	polymer	621	70749.6	1	UniProt (A0A0H3AJ64)	Vibrio cholerae O395	Sigma-70
9	L, M (D)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
10	N (D)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

H, K: 1 - 115 (UniProt: A0A0H3AHV1)

PDB	External Database	Details
Ala 19	Cys 19	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	11
	Total formula weight	565898.6
Non-Polymers*	Number of molecules	3
	Total formula weight	155.1
All*	Total formula weight	566053.7

*Water molecules are not included.