9G9M
Lipid III flippase WzxE with NB10 and NB7 nanobodies in outward-facing conformation - crystal 1
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Lipid III flippase | polymer | 425 | 46130.6 | 1 | UniProt (P0AAA7) Pfam (PF01943) | Escherichia coli | |
| 2 | B (B) | NB10 Nanobody | polymer | 140 | 15132.8 | 1 | Lama glama | ||
| 3 | C (C) | NB7 Nanobody | polymer | 136 | 15223.9 | 1 | Lama glama | ||
| 4 | D (A) | PHOSPHATE ION | non-polymer | 95.0 | 1 | Chemie (PO4) | |||
| 5 | E, F, G (A, B, C) | water | water | 18.0 | 38 | Chemie (HOH) |
Sequence modifications
A: 2 - 416 (UniProt: P0AAA7)
| PDB | External Database | Details |
|---|---|---|
| Met 0 | - | initiating methionine |
| Val 1 | - | cloning artifact |
| Leu 417 | - | expression tag |
| Glu 418 | - | expression tag |
| Glu 419 | - | expression tag |
| Asn 420 | - | expression tag |
| Leu 421 | - | expression tag |
| Tyr 422 | - | expression tag |
| Phe 423 | - | expression tag |
| Gln 424 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 76487.3 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 95.0 | |
| All* | Total formula weight | 76582.2 |
