9G9M
Lipid III flippase WzxE with NB10 and NB7 nanobodies in outward-facing conformation - crystal 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-07 |
| Detector | DECTRIS EIGER2 X CdTe 9M |
| Wavelength(s) | 0.6199 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 38.855, 152.687, 287.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.520 - 2.550 |
| R-factor | 0.2373 |
| Rwork | 0.235 |
| R-free | 0.28700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.403 |
| Data reduction software | xia2 |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.520 | 2.600 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Number of reflections | 28454 | 1280 |
| <I/σ(I)> | 5.9 | 0.6 |
| Completeness [%] | 99.2 | 89.4 |
| Redundancy | 6.2 | 4.1 |
| CC(1/2) | 1.000 | 0.300 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 5 | 293 | tri-sodium citrate, ammonium acetate, 1-propanol, PEG400, E. coli polar lipids, monoolein |
