9G4N

Glycoside Hydrolase Family 157 from Labilibaculum antarcticum (LaGH157) E224A mutant in complex with Laminaritriose and Glucose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Glycoside hydrolase family 2 catalytic domain-containing protein	polymer	537	61456.0	4	UniProt (A0A1Y1CQ89)	Labilibaculum antarcticum
2	E, F, G, H (E, F, G, H)	beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-3)-beta-D-glucopyranose	branched		504.4	4	In PDB GlyTouCan (G00024MO)
3	I, L, O, R, W (A, B, C, D)	1,2-ETHANEDIOL	non-polymer		62.1	5	Chemie (EDO)
4	J, M, U (A, B, D)	beta-D-glucopyranose	non-polymer		180.2	3	Chemie (BGC)
5	AA, K, N, P, Q... (D, A, B, C)	GLYCEROL	non-polymer		92.1	10	Chemie (GOL)
6	S (C)	TRIETHYLENE GLYCOL	non-polymer		150.2	1	Chemie (PGE)
7	BA, CA, DA, EA (A, B, C, D)	water	water		18.0	969	Chemie (HOH)

Sequence modifications

A, B, C, D: 2 - 529 (UniProt: A0A1Y1CQ89)

PDB	External Database	Details
Met 1	-	initiating methionine
Ala 224	Glu 244	engineered mutation
Leu 530	-	expression tag
Glu 531	-	expression tag
His 532	-	expression tag
His 533	-	expression tag
His 534	-	expression tag
His 535	-	expression tag
His 536	-	expression tag
His 537	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	245824.1
Branched	Number of molecules	4
	Total formula weight	2017.8
Non-Polymers*	Number of molecules	19
	Total formula weight	1921.9
All*	Total formula weight	249763.7

*Water molecules are not included.