9G3W

Human Gamma-D crystallin R36S mutant with TNB-Cystein Protein modification

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (X, A)	Gamma-crystallin D	polymer	194	22883.4	2	UniProt (P07320) Pfam (PF00030)	Homo sapiens (human)	Gamma-D-crystallin,Gamma-crystallin 4
2	C, D, E, F, G... (X, A)	5-MERCAPTO-2-NITRO-BENZOIC ACID	non-polymer		199.2	6	Chemie (MNB)
3	I, J (X, A)	water	water		18.0	100	Chemie (HOH)

Sequence modifications

X, A: 0 - 173 (UniProt: P07320)

PDB	External Database	Details
Met -20	-	initiating methionine
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Val -6	-	expression tag
Pro -5	-	expression tag
Arg -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Ser 36	Arg 37	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	45766.8
Non-Polymers*	Number of molecules	6
	Total formula weight	1195.1
All*	Total formula weight	46961.9

*Water molecules are not included.