9G3W
Human Gamma-D crystallin R36S mutant with TNB-Cystein Protein modification
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SwissFEL ARAMIS BEAMLINE ESC |
| Synchrotron site | SwissFEL ARAMIS |
| Beamline | ESC |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2024-03-23 |
| Detector | PSI JUNGFRAU 8M |
| Wavelength(s) | 1.02 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.800, 83.500, 100.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.670 - 1.800 |
| R-factor | 0.20199 |
| Rwork | 0.200 |
| R-free | 0.24334 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.652 |
| Data reduction software | CrystFEL |
| Data scaling software | CrystFEL |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.633 | 1.900 |
| High resolution limit [Å] | 1.152 | 1.800 |
| Number of reflections | 115699 | 4605 |
| <I/σ(I)> | 1.17 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 139 | 131 |
| CC(1/2) | 0.957 | 0.180 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 7.8 | 298 | 50 mM phosphate buffer pH 7.8 |
