9G3T
Structure of the PRO-PRO endopeptidase (PPEP-3) E153A Y189F from Geobacillus thermodenitrificans
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | ATLF-like domain-containing protein | polymer | 230 | 26307.5 | 2 | UniProt (A4INY2) Pfam (PF07737) | Geobacillus thermodenitrificans NG80-2 | |
| 2 | C, G (A, B) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 3 | D, H (A, B) | BICINE | non-polymer | 163.2 | 2 | Chemie (BCN) | |||
| 4 | E (A) | TETRAETHYLENE GLYCOL | non-polymer | 194.2 | 1 | Chemie (PG4) | |||
| 5 | F, I, J (B) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 3 | Chemie (PEG) | |||
| 6 | K, L (A, B) | water | water | 18.0 | 254 | Chemie (HOH) |
Sequence modifications
A, B: 27 - 235 (UniProt: A4INY2)
| PDB | External Database | Details |
|---|---|---|
| Met 6 | - | initiating methionine |
| Gly 7 | - | expression tag |
| Ser 8 | - | expression tag |
| Ser 9 | - | expression tag |
| His 10 | - | expression tag |
| His 11 | - | expression tag |
| His 12 | - | expression tag |
| His 13 | - | expression tag |
| His 14 | - | expression tag |
| His 15 | - | expression tag |
| Ser 16 | - | expression tag |
| Ser 17 | - | expression tag |
| Gly 18 | - | expression tag |
| Leu 19 | - | expression tag |
| Val 20 | - | expression tag |
| Pro 21 | - | expression tag |
| Arg 22 | - | expression tag |
| Gly 23 | - | expression tag |
| Ser 24 | - | expression tag |
| His 25 | - | expression tag |
| Met 26 | - | expression tag |
| Ala 154 | Glu 154 | engineered mutation |
| Phe 190 | Tyr 190 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 52615.0 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 969.7 | |
| All* | Total formula weight | 53584.7 |
