9G3T

Structure of the PRO-PRO endopeptidase (PPEP-3) E153A Y189F from Geobacillus thermodenitrificans

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	ATLF-like domain-containing protein	polymer	230	26307.5	2	UniProt (A4INY2)	Geobacillus thermodenitrificans NG80-2
2	C, G (A, B)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
3	D, H (A, B)	BICINE	non-polymer		163.2	2	Chemie (BCN)
4	E (A)	TETRAETHYLENE GLYCOL	non-polymer		194.2	1	Chemie (PG4)
5	F, I, J (B)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	3	Chemie (PEG)
6	K, L (A, B)	water	water		18.0	254	Chemie (HOH)

Sequence modifications

A, B: 27 - 235 (UniProt: A4INY2)

PDB	External Database	Details
Met 6	-	initiating methionine
Gly 7	-	expression tag
Ser 8	-	expression tag
Ser 9	-	expression tag
His 10	-	expression tag
His 11	-	expression tag
His 12	-	expression tag
His 13	-	expression tag
His 14	-	expression tag
His 15	-	expression tag
Ser 16	-	expression tag
Ser 17	-	expression tag
Gly 18	-	expression tag
Leu 19	-	expression tag
Val 20	-	expression tag
Pro 21	-	expression tag
Arg 22	-	expression tag
Gly 23	-	expression tag
Ser 24	-	expression tag
His 25	-	expression tag
Met 26	-	expression tag
Ala 154	Glu 154	engineered mutation
Phe 190	Tyr 190	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	52615.0
Non-Polymers*	Number of molecules	8
	Total formula weight	969.7
All*	Total formula weight	53584.7

*Water molecules are not included.