9G0A
Influenza A/H7N9 polymerase post-cleavage cap-snatching complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (Z) | Transcription elongation factor SPT5 | polymer | 1087 | 121145.5 | 1 | UniProt (O00267) | Homo sapiens (human) | hSPT5,DRB sensitivity-inducing factor 160 kDa subunit,DSIF p160,DRB sensitivity-inducing factor large subunit,DSIF large subunit,Tat-cotransactivator 1 protein,Tat-CT1 protein |
| 2 | B (Y) | Transcription elongation factor SPT4 | polymer | 117 | 13210.2 | 1 | UniProt (P63272) | Homo sapiens (human) | hSPT4,DRB sensitivity-inducing factor 14 kDa subunit,DSIF p14,DRB sensitivity-inducing factor small subunit,DSIF small subunit |
| 3 | C (A) | DNA-directed RNA polymerase subunit | polymer | 1641 | 183244.3 | 1 | UniProt (I3LJR4) | Sus scrofa domesticus (domestic pig) | |
| 4 | D (P) | 5' cap(1) RNA | polymer | 35 | 11437.0 | 1 | Influenza B virus | ||
| 5 | E (a) | Polymerase acidic protein | polymer | 717 | 82916.6 | 1 | UniProt (M9TI86) | Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)) | RNA-directed RNA polymerase subunit P2 |
| 6 | F (b) | RNA-directed RNA polymerase catalytic subunit | polymer | 757 | 86496.2 | 1 | UniProt (M9TLW3) | Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)) | Polymerase basic protein 1,PB1,RNA-directed RNA polymerase subunit P1 |
| 7 | G (c) | Polymerase basic protein 2 | polymer | 759 | 86007.3 | 1 | UniProt (X5F427) | Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)) | RNA-directed RNA polymerase subunit P3 |
| 8 | H (r) | 3' vRNA | polymer | 13 | 4008.4 | 1 | Influenza B virus | ||
| 9 | I (v) | 5' vRNA | polymer | 14 | 4557.8 | 1 | Influenza B virus | ||
| 10 | J (X) | likely linker between KOWx4 and KOW5 domains of SPT5 | polymer | 14 | 1089.2 | 1 | Homo sapiens | ||
| 11 | K (B) | DNA-directed RNA polymerase subunit beta | polymer | 1174 | 134041.4 | 1 | UniProt (A0A0B8RVL1) | Sus scrofa domesticus (domestic pig) | |
| 12 | L (C) | DNA-directed RNA polymerase II subunit RPB3 | polymer | 275 | 31439.1 | 1 | UniProt (I3LCH3) | Sus scrofa domesticus (domestic pig) | RNA polymerase II subunit C |
| 13 | M (E) | DNA-directed RNA polymerase II subunit E | polymer | 210 | 24644.3 | 1 | UniProt (I3LSI7) | Sus scrofa domesticus (domestic pig) | RPB5 homolog |
| 14 | N (F) | DNA-directed RNA polymerases I, II, and III subunit RPABC2 | polymer | 127 | 14477.0 | 1 | UniProt (A0A4X1VEK9) | Sus scrofa domesticus (domestic pig) | DNA-directed RNA polymerase II subunit F,RPB6 homolog |
| 15 | O (H) | DNA-directed RNA polymerases I, II, and III subunit RPABC3 | polymer | 150 | 17162.3 | 1 | UniProt (I3LCB2) | Sus scrofa domesticus (domestic pig) | |
| 16 | P (I) | DNA-directed RNA polymerase II subunit RPB9 | polymer | 125 | 14541.2 | 1 | UniProt (P60899) | Sus scrofa domesticus (domestic pig) | RNA polymerase II subunit B9,DNA-directed RNA polymerase II subunit I,RNA polymerase II 14.5 kDa subunit,RPB14.5 |
| 17 | Q (J) | DNA-directed RNA polymerases I, II, and III subunit RPABC5 | polymer | 67 | 7655.1 | 1 | UniProt (A0A4X1VYD0) | Sus scrofa domesticus (domestic pig) | |
| 18 | R (K) | DNA-directed RNA polymerase II subunit RPB11-a | polymer | 117 | 13310.3 | 1 | UniProt (F1RKE4) | Sus scrofa domesticus (domestic pig) | |
| 19 | S (L) | RNA polymerase II, I and III subunit K | polymer | 58 | 7018.2 | 1 | UniProt (A0A4X1TRS6) | Sus scrofa domesticus (domestic pig) | |
| 20 | T (N) | nontemplate DNA (43-mer) | polymer | 43 | 13303.6 | 1 | synthetic construct | ||
| 21 | U (T) | template DNA (43-mer) | polymer | 43 | 13193.5 | 1 | synthetic construct | ||
| 22 | V (D) | RNA polymerase II subunit D | polymer | 184 | 20962.6 | 1 | UniProt (A0A287ADR4) | Sus scrofa domesticus (domestic pig) | |
| 23 | W (G) | DNA-directed RNA polymerase subunit | polymer | 172 | 19314.3 | 1 | UniProt (A0A4X1VKG7) | Sus scrofa domesticus (domestic pig) | |
| 24 | FA, GA, HA, IA, JA... (B, C, I, J, L...) | ZINC ION | non-polymer | 65.4 | 8 | Chemie (ZN) | |||
| 25 | BA, Z (a, A) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 26 | AA (P) | 7-METHYL-GUANOSINE-5'-TRIPHOSPHATE-5'-(2'-O-METHYL)-GUANOSINE | non-polymer | 817.5 | 1 | Chemie (G1G) | |||
| 27 | CA, DA, EA (a, c) | PHOSPHATE ION | non-polymer | 95.0 | 3 | Chemie (PO4) |
Sequence modifications
A: 11 - 3022 (UniProt: I3LJR4)
a: 1 - 716 (UniProt: M9TI86)
| PDB | External Database | Details |
|---|---|---|
| Thr 527 | - | insertion |
| Gly 1300 | - | insertion |
| PDB | External Database | Details |
|---|---|---|
| Gly 0 | - | expression tag |
| Asp 119 | Glu 119 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 23 |
| Total formula weight | 925175.3 | |
| Non-Polymers* | Number of molecules | 14 |
| Total formula weight | 1674.3 | |
| All* | Total formula weight | 926849.6 |
