9FL9

Cryo-EM structure of the human KEOPS complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	EKC/KEOPS complex subunit TPRKB	polymer	188	21364.7	1	UniProt (Q9Y3C4) Pfam (PF08617)	Homo sapiens (human)	PRPK-binding protein,TP53RK-binding protein
2	B (B)	EKC/KEOPS complex subunit TP53RK	polymer	266	29885.3	1	UniProt (Q96S44) Pfam (PF06293)	Homo sapiens (human)	Atypical serine/threonine protein kinase TP53RK,Nori-2,TP53-regulating kinase,p53-related protein kinase
3	C (C)	Probable tRNA N6-adenosine threonylcarbamoyltransferase	polymer	335	36466.8	1	UniProt (Q9NPF4) Pfam (PF00814)	Homo sapiens (human)	N6-L-threonylcarbamoyladenine synthase,t(6)A synthase,O-sialoglycoprotein endopeptidase,hOSGEP,t(6)A37 threonylcarbamoyladenosine biosynthesis protein OSGEP,tRNA threonylcarbamoyladenosine biosynthesis protein OSGEP
4	D (D)	EKC/KEOPS complex subunit LAGE3	polymer	143	14825.8	1	UniProt (Q14657) Pfam (PF09341)	Homo sapiens (human)	L antigen family member 3,Protein ESO-3,Protein ITBA2
5	E (E)	EKC/KEOPS complex subunit GON7	polymer	106	11697.6	1	UniProt (Q9BXV9) Pfam (PF15387)	Homo sapiens (human)

Sequence modifications

A: 2 - 175 (UniProt: Q9Y3C4)

PDB	External Database	Details
Met -12	-	initiating methionine
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
Glu -5	-	expression tag
Asn -4	-	expression tag
Leu -3	-	expression tag
Tyr -2	-	expression tag
Phe -1	-	expression tag
Gln 0	-	expression tag
Gly 1	-	expression tag

B: 2 - 253 (UniProt: Q96S44)

PDB	External Database	Details
Met -12	-	initiating methionine
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
Glu -5	-	expression tag
Asn -4	-	expression tag
Leu -3	-	expression tag
Tyr -2	-	expression tag
Phe -1	-	expression tag
Gln 0	-	expression tag
Gly 1	-	expression tag

E: 1 - 100 (UniProt: Q9BXV9)

PDB	External Database	Details
His 101	-	expression tag
His 102	-	expression tag
His 103	-	expression tag
His 104	-	expression tag
His 105	-	expression tag
His 106	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	114240.2
All*	Total formula weight	114240.2

*Water molecules are not included.