9FIZ

Structure of the F13 protein (mutant A295E) of Vaccinia virus

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Envelope phospholipase OPG057	polymer	378	42324.8	1	UniProt (P04021) Pfam (PF00614) Pfam (PF13091)	Vaccinia virus Western Reserve	37 kDa protein,Envelope phospholipase F13,Palmitoylated EV membrane protein,p37K
2	B (A)	CITRIC ACID	non-polymer		192.1	1	Chemie (CIT)
3	C, D, E, F, G... (A)	GLYCEROL	non-polymer		92.1	13	Chemie (GOL)
4	P (A)	water	water		18.0	59	Chemie (HOH)

Sequence modifications

A: 6 - 372 (UniProt: P04021)

PDB	External Database	Details
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
His 2	-	expression tag
Gly 3	-	expression tag
Ser 4	-	expression tag
Gly 5	-	expression tag
Ala 177	Trp 177	conflict
Ala 178	Leu 178	conflict
Ala 181	Cys 181	conflict
Ala 185	Cys 185	conflict
Ala 186	Cys 186	conflict
Glu 295	Ala 295	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	42324.8
Non-Polymers*	Number of molecules	14
	Total formula weight	1389.3
All*	Total formula weight	43714.2

*Water molecules are not included.