9FIZ

Structure of the F13 protein (mutant A295E) of Vaccinia virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-11-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978565
Spacegroup name	F 4 3 2
Unit cell lengths	280.979, 280.979, 280.979
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.670 - 2.600
R-factor	0.218
Rwork	0.216
R-free	0.25860
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.484
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.670	2.693
High resolution limit [Å]	2.600	2.600
Number of reflections	29768	2921
<I/σ(I)>	21.2
Completeness [%]	96.1
Redundancy	80.7
CC(1/2)	1.000	0.644

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	1 M Na3 citrate, 0.1 M Imidazole pH 8 (cryoprotected with 33% glycerol)