9FGB

Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in SMALPs bound to one PIP2 molecule at chain alpha1-A and in complex with Mb38

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, D)	Gamma-aminobutyric acid receptor subunit alpha-1	polymer	464	52916.6	2	UniProt (P14867)	Homo sapiens (human)	GABA(A) receptor subunit alpha-1
2	B, E (B, E)	Gamma-aminobutyric acid receptor subunit beta-3	polymer	473	54444.6	2	UniProt (P28472)	Homo sapiens (human)	GABA(A) receptor subunit beta-3
3	C (C)	Gamma-aminobutyric acid receptor subunit gamma-2	polymer	495	56922.1	1	UniProt (P18507)	Homo sapiens (human)	GABA(A) receptor subunit gamma-2
4	F (F)	Megabody-38,Outer membrane protein	polymer	539	57784.3	1	UniProt (B5Z8H1)	Lama glama
5	G (a)	alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1721.5	1	In PDB GlyTouCan (G40702WU)
6	H, K (b, e)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	2	In PDB GlyTouCan (G42666HT)
7	I, L (G, H)	alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1073.0	2	In PDB GlyTouCan (G09724ZC)
8	J (d)	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		910.8	1	In PDB GlyTouCan (G22768VO)
9	M (A)	[(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate	non-polymer		746.6	1	Chemie (PIO)
10	N (C)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)

Sequence modifications

A, D: 1 - 429 (UniProt: P14867)

PDB	External Database	Details
Met -34	-	initiating methionine
Lys -33	-	expression tag
Lys -32	-	expression tag
Ser -31	-	expression tag
Pro -30	-	expression tag
Gly -29	-	expression tag
Leu -28	-	expression tag
Ser -27	-	expression tag
Asp -26	-	expression tag
Tyr -25	-	expression tag
Leu -24	-	expression tag
Trp -23	-	expression tag
Ala -22	-	expression tag
Trp -21	-	expression tag
Thr -20	-	expression tag
Leu -19	-	expression tag
Phe -18	-	expression tag
Leu -17	-	expression tag
Ser -16	-	expression tag
Thr -15	-	expression tag
Leu -14	-	expression tag
Thr -13	-	expression tag
Gly -12	-	expression tag
Arg -11	-	expression tag
Ser -10	-	expression tag
Tyr -9	-	expression tag
Gly -8	-	expression tag
Asp -7	-	expression tag
Tyr -6	-	expression tag
Lys -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Lys 0	-	expression tag

C: -38 - 436 (UniProt: P18507)

PDB	External Database	Details
Gly 437	-	expression tag
Gly 438	-	expression tag
Ser 439	-	expression tag
Gly 440	-	expression tag
Gly 441	-	expression tag
Ser 442	-	expression tag
Gly 443	-	expression tag
Gly 444	-	expression tag
Ser 445	-	expression tag
Gly 446	-	expression tag
Lys 447	-	expression tag
Thr 448	-	expression tag
Glu 449	-	expression tag
Thr 450	-	expression tag
Ser 451	-	expression tag
Gln 452	-	expression tag
Val 453	-	expression tag
Ala 454	-	expression tag
Pro 455	-	expression tag
Ala 456	-	expression tag

F: 2 - 13 (PDB: 9FGB)
F: 14 - 237 (UniProt: B5Z8H1)
F: 238 - 540 (PDB: 9FGB)

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	329428.7
Branched	Number of molecules	6
	Total formula weight	5627.1
Non-Polymers*	Number of molecules	2
	Total formula weight	967.8
All*	Total formula weight	336023.6

*Water molecules are not included.