9FE1

Cryo-EM structure of the ternary DARPin NY_1/HLA-A0201/NY-ESO1 complex.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	MHC class I antigen	polymer	291	33541.0	1	UniProt (Q8WLS4) Pfam (PF00129) Pfam (PF07654)	Homo sapiens (human)
2	B (B)	Beta-2-microglobulin	polymer	100	11879.4	1	UniProt (P61769) Pfam (PF07654)	Homo sapiens (human)
3	C (C)	Cancer/testis antigen 1	polymer	9	1090.3	1	UniProt (P78358)	Homo sapiens	Autoimmunogenic cancer/testis antigen NY-ESO-1,Cancer/testis antigen 6.1,CT6.1,L antigen family member 2,LAGE-2
4	D (D)	DARPin NY_1	polymer	175	18596.2	1		synthetic construct

Sequence modifications

A: 1 - 276 (UniProt: Q8WLS4)

PDB	External Database	Details
Met 0	-	initiating methionine
Gly 277	-	expression tag
Ser 278	-	expression tag
Gly 279	-	expression tag
Gly 280	-	expression tag
Ser 281	-	expression tag
Ala 282	-	expression tag
Trp 283	-	expression tag
Ser 284	-	expression tag
His 285	-	expression tag
Pro 286	-	expression tag
Gln 287	-	expression tag
Phe 288	-	expression tag
Glu 289	-	expression tag
Lys 290	-	expression tag

B: 1 - 99 (UniProt: P61769)

PDB	External Database	Details
Met 0	-	initiating methionine

C: 1 - 9 (UniProt: P78358)

PDB	External Database	Details
Val 9	Cys 165	conflict

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	65107.0
All*	Total formula weight	65107.0

*Water molecules are not included.