9FD2

Structure of Pol II-TC-NER-STK19 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA-directed RNA polymerase subunit	polymer	1970	217450.1	1	UniProt (A0A8D1DPV6) Pfam (PF04997) Pfam (PF05001) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) Pfam (PF04992) Pfam (PF04990)	Sus scrofa domesticus (domestic pig)
2	B (B)	DNA-directed RNA polymerase subunit beta	polymer	1300	147938.6	1	UniProt (I3LGP4) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF04566) Pfam (PF04567) Pfam (PF00562) Pfam (PF04560)	Sus scrofa domesticus (domestic pig)
3	C (C)	DNA-directed RNA polymerase II subunit RPB3	polymer	275	31439.1	1	UniProt (I3LCH3) Pfam (PF01193) Pfam (PF01000)	Sus scrofa domesticus (domestic pig)	RNA polymerase II subunit C
4	D (D)	RNA polymerase II subunit D	polymer	142	16331.3	1	UniProt (A0A4X1VM56) Pfam (PF03874)	Sus scrofa domesticus (domestic pig)
5	E (E)	DNA-directed RNA polymerase II subunit E	polymer	210	24644.3	1	UniProt (I3LSI7) Pfam (PF03871) Pfam (PF01191)	Sus scrofa domesticus (domestic pig)	RPB5 homolog
6	F (F)	DNA-directed RNA polymerases I, II, and III subunit RPABC2	polymer	127	14477.0	1	UniProt (A0A4X1VEK9) Pfam (PF01192)	Sus scrofa domesticus (domestic pig)	DNA-directed RNA polymerase II subunit F,RPB6 homolog
7	G (G)	DNA-directed RNA polymerase subunit	polymer	172	19314.3	1	UniProt (A0A4X1VKG7) Pfam (PF03876) Pfam (PF00575)	Sus scrofa domesticus (domestic pig)
8	H (H)	DNA-directed RNA polymerases I, II, and III subunit RPABC3	polymer	150	17162.3	1	UniProt (I3LCB2) Pfam (PF03870)	Sus scrofa domesticus (domestic pig)
9	I (I)	DNA-directed RNA polymerase II subunit RPB9	polymer	125	14541.2	1	UniProt (P60899) Pfam (PF02150) Pfam (PF01096)	Sus scrofa domesticus (domestic pig)	RNA polymerase II subunit B9,DNA-directed RNA polymerase II subunit I,RNA polymerase II 14.5 kDa subunit,RPB14.5
10	J (J)	DNA-directed RNA polymerases I, II, and III subunit RPABC5	polymer	67	7655.1	1	UniProt (A0A4X1VYD0) Pfam (PF01194) UniProt (by SIFTS) (A0A8W4F9W9)	Sus scrofa domesticus (domestic pig)
11	K (K)	DNA-directed RNA polymerase II subunit RPB11-a	polymer	117	13310.3	1	UniProt (F1RKE4) Pfam (PF13656)	Sus scrofa domesticus (domestic pig)
12	L (L)	RNA polymerase II, I and III subunit K	polymer	58	7018.2	1	UniProt (A0A4X1TRS6) Pfam (PF03604)	Sus scrofa domesticus (domestic pig)
13	M (f)	Transcription elongation factor 1 homolog	polymer	85	9604.0	1	UniProt (P60002) Pfam (PF05129)	Homo sapiens (human)
14	N (N)	Non-template DNA	polymer	60	18496.9	1		Homo sapiens
15	O (M)	RNA	polymer	25	8101.9	1		Homo sapiens
16	P (T)	Template DNA	polymer	60	18298.7	1		Homo sapiens
17	Q (a)	DNA excision repair protein ERCC-8	polymer	408	45465.6	1	UniProt (Q13216) Pfam (PF00400)	Homo sapiens (human)	Cockayne syndrome WD repeat protein CSA
18	R (b)	DNA damage-binding protein 1	polymer	1160	129298.9	1	UniProt (Q16531) Pfam (PF10433) Pfam (PF03178)	Homo sapiens (human)	DDB p127 subunit,DNA damage-binding protein a,DDBa,Damage-specific DNA-binding protein 1,HBV X-associated protein 1,XAP-1,UV-damaged DNA-binding factor,UV-damaged DNA-binding protein 1,UV-DDB 1,XPE-binding factor,XPE-BF,Xeroderma pigmentosum group E-complementing protein,XPCe
19	S (c)	DET1- and DDB1-associated protein 1	polymer	152	16997.6	1	UniProt (Q9BW61) Pfam (PF10172)	Homo sapiens (human)	Placenta cross-immune reaction antigen 1,PCIA-1
20	T (g)	Inactive serine/threonine-protein kinase 19	polymer	257	28717.2	1	UniProt (P49842) Pfam (PF10494)	Homo sapiens (human)	Protein G11,Protein RP1
21	U (e)	DNA excision repair protein ERCC-6	polymer	1493	168673.5	1	UniProt (Q03468) Pfam (PF00176) Pfam (PF00271)	Homo sapiens (human)	ATP-dependent helicase ERCC6,Cockayne syndrome protein CSB
22	V (d)	UV-stimulated scaffold protein A	polymer	729	82923.2	1	UniProt (Q2YD98) Pfam (PF20867) Pfam (PF09740)	Homo sapiens (human)
23	AA, BA, CA, DA, EA... (C, I, J, L, f...)	ZINC ION	non-polymer		65.4	10	Chemie (ZN)
24	Y (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

f: 1 - 83 (UniProt: P60002)

PDB	External Database	Details
Gly -1	-	expression tag
Ala 0	-	expression tag

a: 1 - 396 (UniProt: Q13216)

PDB	External Database	Details
Gly 397	-	expression tag
Thr 398	-	expression tag
Ser 399	-	expression tag
Ala 400	-	expression tag
Trp 401	-	expression tag
Ser 402	-	expression tag
His 403	-	expression tag
Pro 404	-	expression tag
Gln 405	-	expression tag
Phe 406	-	expression tag
Glu 407	-	expression tag
Lys 408	-	expression tag

b: 1 - 1140 (UniProt: Q16531)

PDB	External Database	Details
Met -19	-	initiating methionine
Ala -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
Ser -11	-	expression tag
Ala -10	-	expression tag
Ala -9	-	expression tag
Leu -8	-	expression tag
Glu -7	-	expression tag
Val -6	-	expression tag
Leu -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
Pro -1	-	expression tag
Gly 0	-	expression tag

c: 1 - 102 (UniProt: Q9BW61)

PDB	External Database	Details
Asp 103	-	expression tag
Val 104	-	expression tag
Leu 105	-	expression tag
Phe 106	-	expression tag
Gln 107	-	expression tag
Gly 108	-	expression tag
Pro 109	-	expression tag
Gly 110	-	expression tag
Ala 111	-	expression tag
Trp 112	-	expression tag
Ser 113	-	expression tag
His 114	-	expression tag
Pro 115	-	expression tag
Gln 116	-	expression tag
Phe 117	-	expression tag
Glu 118	-	expression tag
Lys 119	-	expression tag
Gly 120	-	expression tag
Gly 121	-	expression tag
Gly 122	-	expression tag
Ser 123	-	expression tag
Gly 124	-	expression tag
Gly 125	-	expression tag
Gly 126	-	expression tag
Ser 127	-	expression tag
Gly 128	-	expression tag
Gly 129	-	expression tag
Gly 130	-	expression tag
Ser 131	-	expression tag
Trp 132	-	expression tag
Ser 133	-	expression tag
His 134	-	expression tag
Pro 135	-	expression tag
Gln 136	-	expression tag
Phe 137	-	expression tag
Glu 138	-	expression tag
Lys 139	-	expression tag
Gly 140	-	expression tag
Ala 141	-	expression tag
Ser 142	-	expression tag
Gly 143	-	expression tag
Glu 144	-	expression tag
Asp 145	-	expression tag
Tyr 146	-	expression tag
Lys 147	-	expression tag
Asp 148	-	expression tag
Asp 149	-	expression tag
Asp 150	-	expression tag
Asp 151	-	expression tag
Lys 152	-	expression tag

g: 1 - 254 (UniProt: P49842)

PDB	External Database	Details
Gly -2	-	expression tag
Pro -1	-	expression tag
Gly 0	-	expression tag

d: 1 - 709 (UniProt: Q2YD98)

PDB	External Database	Details
Met -19	-	initiating methionine
Ala -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
Ser -11	-	expression tag
Ala -10	-	expression tag
Ala -9	-	expression tag
Leu -8	-	expression tag
Glu -7	-	expression tag
Val -6	-	expression tag
Leu -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
Pro -1	-	expression tag
Gly 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	22
	Total formula weight	1057859.3
Non-Polymers*	Number of molecules	11
	Total formula weight	678.4
All*	Total formula weight	1058537.7

*Water molecules are not included.