9FBU

X-ray structure of the iGluSnFR3 in complex with L-glutamate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Periplasmic binding transport protein,Green fluorescent protein	polymer	520	58029.4	1	UniProt (A0A0H2UXX1) UniProt (P42212) Pfam (PF00497)	Shigella flexneri
2	B (A)	GLUTAMIC ACID	non-polymer		147.1	1	Chemie (GLU)
3	C (A)	water	water		18.0	334	Chemie (HOH)

Sequence modifications

A: 2 - 250 (UniProt: A0A0H2UXX1)

PDB	External Database	Details
Gly 1	-	expression tag
Asp 22	Glu 48	engineered mutation
Ser 32	Asn 58	engineered mutation
His 34	Gln 60	engineered mutation
Glu 42	Asp 68	engineered mutation
Tyr 81	Phe 107	engineered mutation
Asn 124	Asp 150	engineered mutation
Asp 127	Gly 153	engineered mutation
Ser 181	Ala 207	engineered mutation
Asp 195	Asn 221	engineered mutation
Glu 197	Asp 223	engineered mutation
Leu 249	Pro 275	engineered mutation
Val 250	Pro 276	engineered mutation

A: 251 - 342 (UniProt: P42212)

PDB	External Database	Details
Asn 251	Ser 147	engineered mutation
Thr 253	Asn 149	engineered mutation
Thr 257	Met 153	engineered mutation
Ala 267	Val 163	engineered mutation
His 272	Arg 168	engineered mutation
Val 275	Ile 171	engineered mutation
Tyr 307	Thr 203	engineered mutation
Val 310	Ala 206	engineered mutation
Thr 331	Ala 227	engineered mutation
Asn 333	Ile 229	engineered mutation
Ser 334	Thr 230	engineered mutation
Leu 335	His 231	engineered mutation

A: 349 - 496 (UniProt: P42212)

PDB	External Database	Details
Gly 343	-	linker
Gly 344	-	linker
Thr 345	-	linker
Gly 346	-	linker
Gly 347	-	linker
Ser 348	-	linker
Arg 378	Ser 30	engineered mutation
Asn 387	Tyr 39	engineered mutation
Leu 412	Phe 64	engineered mutation
Gys 413	Ser 65	chromophore
Gys 413	Tyr 66	chromophore
Gys 413	Gly 67	chromophore
Ala 420	Ser 72	engineered mutation
Ser 447	Phe 99	engineered mutation
Thr 453	Asn 105	engineered mutation
Leu 462	Phe 114	engineered mutation
Phe 493	Tyr 145	engineered mutation
Asn 495	Ser 147	engineered mutation
Pro 496	His 148	engineered mutation

A: 497 - 520 (UniProt: A0A0H2UXX1)

PDB	External Database	Details
Lys 520	Asn 302	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	58029.4
Non-Polymers*	Number of molecules	1
	Total formula weight	147.1
All*	Total formula weight	58176.5

*Water molecules are not included.