9FBU

X-ray structure of the iGluSnFR3 in complex with L-glutamate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-06
Detector	DECTRIS EIGER2 X CdTe 16M
Wavelength(s)	0.88560
Spacegroup name	P 21 21 2
Unit cell lengths	68.760, 100.840, 73.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.540 - 1.700
R-factor	0.1842
Rwork	0.183
R-free	0.21070
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.921
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.800
High resolution limit [Å]	1.700	1.700
Rmerge	0.074	0.646
Number of reflections	56404	8736
<I/σ(I)>	14.6	3.2
Completeness [%]	99.3	98.8
Redundancy	6.4	6.2
CC(1/2)	0.998	0.809

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	26% (m/v) PEG 1500