9FAK
CryoEM structure of human full-length alpha1beta3gamma2 GABA(A) receptor in complex with GARLH4, the TMD of Neuroligin2, GABA and Megabody38 in a desensitised state (StateD2)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, D (A, D) | Gamma-aminobutyric acid receptor subunit alpha-1 | polymer | 413 | 47053.1 | 2 | UniProt (P14867) Pfam (PF02931) Pfam (PF02932) | Homo sapiens (human) | GABA(A) receptor subunit alpha-1,GABAAR subunit alpha-1 |
| 2 | B, E (B, E) | Gamma-aminobutyric acid receptor subunit beta-3 | polymer | 441 | 50904.5 | 2 | UniProt (P28472) Pfam (PF02931) Pfam (PF02932) | Homo sapiens (human) | GABA(A) receptor subunit beta-3,GABAAR subunit beta-3 |
| 3 | C (C) | Isoform 2 of Gamma-aminobutyric acid receptor subunit gamma-2 | polymer | 405 | 47870.4 | 1 | UniProt (P18507) Pfam (PF02931) Pfam (PF02932) | Homo sapiens (human) | GABA(A) receptor subunit gamma-2,GABAAR subunit gamma-2 |
| 4 | F (H) | Neuroligin-2 | polymer | 33 | 3614.1 | 1 | UniProt (Q8NFZ4) | Homo sapiens (human) | |
| 5 | G (L) | LHFPL tetraspan subfamily member 4 protein | polymer | 193 | 21366.2 | 1 | UniProt (Q7Z7J7) Pfam (PF10242) | Homo sapiens (human) | GABAA receptor regulatory Lhfpl4,Lipoma HMGIC fusion partner-like 4 protein |
| 6 | H, I (G, P) | Megabody38 | polymer | 539 | 57784.3 | 2 | Lama glama | ||
| 7 | J (F) | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1721.5 | 1 | In PDB GlyTouCan (G40702WU) | |||
| 8 | K, P (I, O) | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 2 | In PDB GlyTouCan (G15407YE) | |||
| 9 | L, O (J, N) | alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1073.0 | 2 | In PDB GlyTouCan (G09724ZC) | |||
| 10 | M (K) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 1 | In PDB GlyTouCan (G42666HT) | |||
| 11 | N (M) | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1235.1 | 1 | In PDB GlyTouCan (G23799GS) | |||
| 12 | BA, BB, CA, PA, Q... (B, E, D, A) | HEXANE | non-polymer | 86.2 | 6 | Chemie (HEX) | |||
| 13 | LA, R (D, A) | [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate | non-polymer | 746.6 | 2 | Chemie (PIO) | |||
| 14 | DB, GB, MA, S, YA (L, D, A, E) | (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate | non-polymer | 749.0 | 5 | Chemie (PGW) | |||
| 15 | GA, NA, SA, T, V (C, D, A) | 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE | non-polymer | 535.7 | 5 | Chemie (PX2) | |||
| 16 | AA, FB, QA, RA, U... (B, L, D, A, E) | DECANE | non-polymer | 142.3 | 7 | Chemie (D10) | |||
| 17 | AB, DA, FA, IA, JA... (E, B, C, D, A) | PALMITIC ACID | non-polymer | 256.4 | 8 | Chemie (PLM) | |||
| 18 | HB, KA, TA, X (L, C, D, A) | CHLORIDE ION | non-polymer | 35.5 | 4 | Chemie (CL) | |||
| 19 | CB, Y (E, B) | GAMMA-AMINO-BUTANOIC ACID | non-polymer | 103.1 | 2 | Chemie (ABU) | |||
| 20 | EA, VA, Z (B, E) | HEXADECANE | non-polymer | 226.4 | 3 | Chemie (R16) | |||
| 21 | HA (C) | (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate | non-polymer | 356.5 | 1 | Chemie (OLC) | |||
| 22 | EB (L) | CHOLESTEROL | non-polymer | 386.7 | 1 | Chemie (CLR) | |||
| 23 | IB, JB, KB, LB, MB... (A, B, C, D, E...) | water | water | 18.0 | 86 | Chemie (HOH) |
Sequence modifications
C: 25 - 428 (UniProt: P18507)
| PDB | External Database | Details |
|---|---|---|
| Gly 429 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 9 |
| Total formula weight | 384334.6 | |
| Branched | Number of molecules | 7 |
| Total formula weight | 6700.0 | |
| Non-Polymers* | Number of molecules | 44 |
| Total formula weight | 13251.5 | |
| All* | Total formula weight | 404286.1 |
