9F6Y

CryoEM structure of Human Mediator subunit MED23 complexed with phosphorylated Elk-1 transcription factor

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Mediator of RNA polymerase II transcription subunit 23	polymer	1382	158294.9	1	UniProt (Q9ULK4) Pfam (PF11573)	Homo sapiens (human)	Activator-recruited cofactor 130 kDa component,ARC130,Cofactor required for Sp1 transcriptional activation subunit 3,CRSP complex subunit 3,Mediator complex subunit 23,Protein sur-2 homolog,hSur-2,Transcriptional coactivator CRSP130,Vitamin D3 receptor-interacting protein complex 130 kDa component,DRIP130
2	B (B)	Green fluorescent protein,ETS domain-containing protein Elk-1	polymer	341	37627.4	1	UniProt (P42212) UniProt (P19419)	Homo sapiens (human)

Sequence modifications

A: 1 - 1368 (UniProt: Q9ULK4)

PDB	External Database	Details
Asp 1369	-	expression tag
Val 1370	-	expression tag
Leu 1371	-	expression tag
Phe 1372	-	expression tag
Gln 1373	-	expression tag
Gly 1374	-	expression tag
Pro 1375	-	expression tag
Gly 1376	-	expression tag
His 1377	-	expression tag
His 1378	-	expression tag
His 1379	-	expression tag
His 1380	-	expression tag
His 1381	-	expression tag
His 1382	-	expression tag

B: 67 - 305 (UniProt: P42212)

PDB	External Database	Details
Val 68	-	insertion
Leu 131	Phe 64	conflict
Thr 132	Ser 65	conflict
Lys 273	Ala 206	conflict
Leu 298	His 231	conflict
Gly 306	-	linker
Ser 307	-	linker

B: 308 - 401 (UniProt: P19419)

PDB	External Database	Details
Ala 336	Thr 336	engineered mutation
Ala 353	Thr 353	engineered mutation
Ala 363	Thr 363	engineered mutation
His 402	-	expression tag
His 403	-	expression tag
His 404	-	expression tag
His 405	-	expression tag
His 406	-	expression tag
His 407	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	195922.3
All*	Total formula weight	195922.3

*Water molecules are not included.