9F5H

Crystal structure of MGAT5 bump-and-hole mutant in complex with UDP and M592

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Secreted alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A	polymer	515	58856.8	2	UniProt (Q09328) In PDB	Homo sapiens (human)	Secreted beta-1,6-N-acetylglucosaminyltransferase V,Secreted GNT-V
2	A, B	URIDINE-5'-DIPHOSPHATE	non-polymer		404.2	2	Chemie (UDP)
3	A, B	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)
4	A	alpha-D-mannopyranose	non-polymer		180.2	2	Chemie (MAN)
5	B	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
6		water	water		18.0	272	Chemie (HOH)

Sequence modifications

A, B: 214 - 328 (UniProt: Q09328)

PDB	External Database	Details
Ala 297	Glu 297	engineered mutation

A, B: 333 - 728 (UniProt: Q09328)

PDB	External Database	Details
Gly 329	-	linker
Gly 330	-	linker
Gly 331	-	linker
Gly 332	-	linker
Val 445	Phe 458	engineered mutation
Leu 504	Phe 517	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	117713.6
Non-Polymers*	Number of molecules	7
	Total formula weight	1707.1
All*	Total formula weight	119420.7

*Water molecules are not included.