9F3S

13pf mosaic 20%E254Q - 80% E254N microtubule from recombinant human tubulin decorated with EB3

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (S, T)	Microtubule-associated protein RP/EB family member 3	polymer	131	15386.8	2	UniProt (Q9UPY8) Pfam (PF00307)	Homo sapiens (human)	EB1 protein family member 3,EBF3,End-binding protein 3,EB3,RP3
2	C, E, G, I, K... (A, G, C, I, E...)	Detyrosinated tubulin alpha-1B chain	polymer	453	50718.2	6	UniProt (P68363) Pfam (PF00091) Pfam (PF03953)	Homo sapiens (human)
3	D, F, H, J, L... (B, N, D, P, F...)	Tubulin beta-3 chain	polymer	456	51276.4	6	UniProt (Q13509) Pfam (PF00091) Pfam (PF03953)	Homo sapiens (human)	Tubulin beta-4 chain,Tubulin beta-III
4	AA, CA, EA, GA, IA... (I, P, E, F, K...)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	12	Chemie (GTP)
5	BA, DA, FA, HA, JA... (I, P, E, F, K...)	MAGNESIUM ION	non-polymer		24.3	12	Chemie (MG)

Sequence modifications

A, G, C, I, E, K: 1 - 37 (UniProt: P68363)
A, G, C, I, E, K: 47 - 441 (UniProt: P68363)

PDB	External Database	Details
His 40	-	linker
His 41	-	linker
His 42	-	linker
His 42	-	linker
His 42	-	linker
His 42	-	linker
Gly 42	-	linker
Gly 42	-	linker
Gly 42	-	linker
His 42	-	linker
His 42	-	linker
His 42	-	linker
Phe 42	-	linker
Asn 42	-	linker
Thr 42	-	linker
Phe 42	-	linker
Asn 254	Glu 254	engineered mutation

B, N, D, P, F, R: 1 - 450 (UniProt: Q13509)

PDB	External Database	Details
Glu 451	-	expression tag
Asn 452	-	expression tag
Leu 453	-	expression tag
Tyr 454	-	expression tag
Phe 455	-	expression tag
Gln 456	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	14
	Total formula weight	642741.1
Non-Polymers*	Number of molecules	24
	Total formula weight	6569.8
All*	Total formula weight	649310.9

*Water molecules are not included.