9F3S
13pf mosaic 20%E254Q - 80% E254N microtubule from recombinant human tubulin decorated with EB3
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (S, T) | Microtubule-associated protein RP/EB family member 3 | polymer | 131 | 15386.8 | 2 | UniProt (Q9UPY8) Pfam (PF00307) | Homo sapiens (human) | EB1 protein family member 3,EBF3,End-binding protein 3,EB3,RP3 |
| 2 | C, E, G, I, K... (A, G, C, I, E...) | Detyrosinated tubulin alpha-1B chain | polymer | 453 | 50718.2 | 6 | UniProt (P68363) Pfam (PF00091) Pfam (PF03953) | Homo sapiens (human) | |
| 3 | D, F, H, J, L... (B, N, D, P, F...) | Tubulin beta-3 chain | polymer | 456 | 51276.4 | 6 | UniProt (Q13509) Pfam (PF00091) Pfam (PF03953) | Homo sapiens (human) | Tubulin beta-4 chain,Tubulin beta-III |
| 4 | AA, CA, EA, GA, IA... (I, P, E, F, K...) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 12 | Chemie (GTP) PubChem (177679032) PubChem (135400627) PubChem (135487750) PubChem (136088935) PubChem (136803486) PubChem (135398633) PubChem (135544356) PubChem (135547949) PubChem (135600366) PubChem (135749822) PubChem (136005188) PubChem (136073923) PubChem (136240914) PubChem (136664747) PubChem (136772743) PubChem (136772744) PubChem (136803487) PubChem (137286749) PubChem (164715984) PubChem (171699466) | |||
| 5 | BA, DA, FA, HA, JA... (I, P, E, F, K...) | MAGNESIUM ION | non-polymer | 24.3 | 12 | Chemie (MG) PubChem (888) |
Sequence modifications
A, G, C, I, E, K: 1 - 37 (UniProt: P68363)
A, G, C, I, E, K: 47 - 441 (UniProt: P68363)
B, N, D, P, F, R: 1 - 450 (UniProt: Q13509)
A, G, C, I, E, K: 47 - 441 (UniProt: P68363)
| PDB | External Database | Details |
|---|---|---|
| His 40 | - | linker |
| His 41 | - | linker |
| His 42 | - | linker |
| His 42 | - | linker |
| His 42 | - | linker |
| His 42 | - | linker |
| Gly 42 | - | linker |
| Gly 42 | - | linker |
| Gly 42 | - | linker |
| His 42 | - | linker |
| His 42 | - | linker |
| His 42 | - | linker |
| Phe 42 | - | linker |
| Asn 42 | - | linker |
| Thr 42 | - | linker |
| Phe 42 | - | linker |
| Asn 254 | Glu 254 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Glu 451 | - | expression tag |
| Asn 452 | - | expression tag |
| Leu 453 | - | expression tag |
| Tyr 454 | - | expression tag |
| Phe 455 | - | expression tag |
| Gln 456 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 14 |
| Total formula weight | 642741.1 | |
| Non-Polymers* | Number of molecules | 24 |
| Total formula weight | 6569.8 | |
| All* | Total formula weight | 649310.9 |
