9F3S
13pf mosaic 20%E254Q - 80% E254N microtubule from recombinant human tubulin decorated with EB3
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS GLACIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Detector | FEI FALCON IV (4k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 4.2 |
| Number of Particle | 54200 |
| Software | RELION,PHENIX,Coot |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
