9F3C
Low-resolution (5.4 angstrom) cryo-EM structure of SV2B-BoNT/A1 at pH 5.5
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Synaptic vesicle glycoprotein 2B | polymer | 683 | 77515.0 | 1 | UniProt (Q7L1I2) Pfam (PF00083) Pfam (PF07690) | Homo sapiens (human) | |
| 2 | B (B) | Botulinum neurotoxin type A | polymer | 1329 | 153083.7 | 1 | UniProt (P0DPI0) Pfam (PF01742) Pfam (PF07952) Pfam (PF07953) Pfam (PF07951) UniProt (by SIFTS) (P0DPI1) | Clostridium botulinum | BoNT/A,Bontoxilysin-A,BOTOX,Botulinum neurotoxin type A1 |
| 3 | C (C) | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 1 | In PDB GlyTouCan (G15407YE) | |||
| 4 | D, E (A) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 2 | Chemie (NAG) |
Sequence modifications
B: 2 - 1296 (UniProt: P0DPI0)
| PDB | External Database | Details |
|---|---|---|
| Met -16 | - | initiating methionine |
| Arg -15 | - | expression tag |
| Gly -14 | - | expression tag |
| Ser -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| Gly -6 | - | expression tag |
| Ser -5 | - | expression tag |
| Leu -4 | - | expression tag |
| Val -3 | - | expression tag |
| Pro -2 | - | expression tag |
| Arg -1 | - | expression tag |
| Gly 0 | - | expression tag |
| Ser 1 | - | expression tag |
| Ala 27 | Val 27 | variant |
| Gln 224 | Glu 224 | engineered mutation |
| Ala 363 | Arg 363 | engineered mutation |
| Phe 366 | Tyr 366 | engineered mutation |
| Ala 1158 | Thr 1158 | conflict |
| Val 1297 | - | expression tag |
| Pro 1298 | - | expression tag |
| Pro 1299 | - | expression tag |
| Thr 1300 | - | expression tag |
| Pro 1301 | - | expression tag |
| Gly 1302 | - | expression tag |
| Ser 1303 | - | expression tag |
| Ala 1304 | - | expression tag |
| Trp 1305 | - | expression tag |
| Ser 1306 | - | expression tag |
| His 1307 | - | expression tag |
| Pro 1308 | - | expression tag |
| Gln 1309 | - | expression tag |
| Phe 1310 | - | expression tag |
| Glu 1311 | - | expression tag |
| Lys 1312 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 230598.8 | |
| Branched | Number of molecules | 1 |
| Total formula weight | 586.5 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 442.4 | |
| All* | Total formula weight | 231627.7 |
