9F3C
Low-resolution (5.4 angstrom) cryo-EM structure of SV2B-BoNT/A1 at pH 5.5
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0001669 | cellular_component | acrosomal vesicle |
| A | 0005515 | molecular_function | protein binding |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0006836 | biological_process | neurotransmitter transport |
| A | 0008021 | cellular_component | synaptic vesicle |
| A | 0016020 | cellular_component | membrane |
| A | 0030672 | cellular_component | synaptic vesicle membrane |
| A | 0031410 | cellular_component | cytoplasmic vesicle |
| A | 0045202 | cellular_component | synapse |
| A | 0099509 | biological_process | regulation of presynaptic cytosolic calcium ion concentration |
| A | 2000300 | biological_process | regulation of synaptic vesicle exocytosis |
| B | 0004222 | molecular_function | metalloendopeptidase activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005576 | cellular_component | extracellular region |
| B | 0006508 | biological_process | proteolysis |
| B | 0008233 | molecular_function | peptidase activity |
| B | 0008237 | molecular_function | metallopeptidase activity |
| B | 0008270 | molecular_function | zinc ion binding |
| B | 0008289 | molecular_function | lipid binding |
| B | 0008320 | molecular_function | protein transmembrane transporter activity |
| B | 0016020 | cellular_component | membrane |
| B | 0016787 | molecular_function | hydrolase activity |
| B | 0020002 | cellular_component | host cell plasma membrane |
| B | 0030430 | cellular_component | host cell cytoplasm |
| B | 0033644 | cellular_component | host cell membrane |
| B | 0044161 | cellular_component | host cell cytoplasmic vesicle |
| B | 0044164 | cellular_component | host cell cytosol |
| B | 0044221 | cellular_component | host cell synapse |
| B | 0044231 | cellular_component | host cell presynaptic membrane |
| B | 0046872 | molecular_function | metal ion binding |
| B | 0071806 | biological_process | protein transmembrane transport |
| B | 0090729 | molecular_function | toxin activity |
| B | 0141157 | biological_process | symbiont-mediated suppression of host exocytosis |
| B | 1905576 | molecular_function | ganglioside GT1b binding |
Functional Information from PROSITE/UniProt
| site_id | PS00216 |
| Number of Residues | 17 |
| Details | SUGAR_TRANSPORT_1 Sugar transport proteins signature 1. LGGLADKLGRKrvlsm.S |
| Chain | Residue | Details |
| A | LEU165-SER181 |
| site_id | PS00217 |
| Number of Residues | 26 |
| Details | SUGAR_TRANSPORT_2 Sugar transport proteins signature 2. IsGIGiGGalpivfaYfsEflsrekR |
| Chain | Residue | Details |
| A | ILE207-ARG232 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 280 |
| Details | Transmembrane: {"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 42 |
| Details | Topological domain: {"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 27 |
| Details | Topological domain: {"description":"Extracellular","evidences":[{"evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"Phosphotyrosine","evidences":[{"source":"UniProtKB","id":"Q8BG39","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 3 |
| Details | Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 221 |
| Details | Region: {"description":"N-terminus of receptor binding domain (N-RBD)","evidences":[{"source":"PubMed","id":"19096517","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 1 |
| Details | Region: {"description":"Interaction with host ganglioside GT1b","evidences":[{"source":"PubMed","id":"18704164","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27958736","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"14731268","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"2VU9","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5TPB","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5TPC","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 1 |
| Details | Active site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU10095","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI9 |
| Number of Residues | 2 |
| Details | Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU10095","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"17173035","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9783750","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"2NYY","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2NZ9","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3BTA","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3QIX","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3QIY","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3QIZ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3QJ0","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI10 |
| Number of Residues | 1 |
| Details | Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU10095","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"17173035","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"2NYY","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2NZ9","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3BTA","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3QIX","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3QIY","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3QIZ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3QJ0","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI11 |
| Number of Residues | 1 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"18704164","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27958736","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"2VU9","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5TPB","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5TPC","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI12 |
| Number of Residues | 1 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"18704164","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27958736","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"14731268","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"2VU9","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5TPB","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5TPC","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI13 |
| Number of Residues | 2 |
| Details | Site: {"description":"Transition state stabilizer","evidences":[{"source":"PubMed","id":"11827515","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI14 |
| Number of Residues | 219 |
| Details | Region: {"description":"N-terminus of receptor binding domain (N-RBD)","evidences":[{"source":"PubMed","id":"17173035","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"9783750","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
