9F3C
Low-resolution (5.4 angstrom) cryo-EM structure of SV2B-BoNT/A1 at pH 5.5
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0001669 | cellular_component | acrosomal vesicle |
| A | 0005515 | molecular_function | protein binding |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0006836 | biological_process | neurotransmitter transport |
| A | 0008021 | cellular_component | synaptic vesicle |
| A | 0016020 | cellular_component | membrane |
| A | 0030672 | cellular_component | synaptic vesicle membrane |
| A | 0031410 | cellular_component | cytoplasmic vesicle |
| A | 0045202 | cellular_component | synapse |
| A | 0099509 | biological_process | regulation of presynaptic cytosolic calcium ion concentration |
| A | 2000300 | biological_process | regulation of synaptic vesicle exocytosis |
| B | 0004222 | molecular_function | metalloendopeptidase activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005576 | cellular_component | extracellular region |
| B | 0006508 | biological_process | proteolysis |
| B | 0008233 | molecular_function | peptidase activity |
| B | 0008237 | molecular_function | metallopeptidase activity |
| B | 0008270 | molecular_function | zinc ion binding |
| B | 0008289 | molecular_function | lipid binding |
| B | 0008320 | molecular_function | protein transmembrane transporter activity |
| B | 0016020 | cellular_component | membrane |
| B | 0016787 | molecular_function | hydrolase activity |
| B | 0020002 | cellular_component | host cell plasma membrane |
| B | 0030430 | cellular_component | host cell cytoplasm |
| B | 0033644 | cellular_component | host cell membrane |
| B | 0044161 | cellular_component | host cell cytoplasmic vesicle |
| B | 0044164 | cellular_component | host cell cytosol |
| B | 0044221 | cellular_component | host cell synapse |
| B | 0044231 | cellular_component | host cell presynaptic membrane |
| B | 0046872 | molecular_function | metal ion binding |
| B | 0071806 | biological_process | protein transmembrane transport |
| B | 0090729 | molecular_function | toxin activity |
| B | 0141157 | biological_process | symbiont-mediated suppression of host exocytosis |
| B | 1905576 | molecular_function | ganglioside GT1b binding |
Functional Information from PROSITE/UniProt
| site_id | PS00216 |
| Number of Residues | 17 |
| Details | SUGAR_TRANSPORT_1 Sugar transport proteins signature 1. LGGLADKLGRKrvlsm.S |
| Chain | Residue | Details |
| A | LEU165-SER181 |
| site_id | PS00217 |
| Number of Residues | 26 |
| Details | SUGAR_TRANSPORT_2 Sugar transport proteins signature 2. IsGIGiGGalpivfaYfsEflsrekR |
| Chain | Residue | Details |
| A | ILE207-ARG232 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 40 |
| Details | TRANSMEM: Helical => ECO:0000255 |
| Chain | Residue | Details |
| B | ILE627-LEU647 | |
| B | LEU656-LEU676 | |
| A | LEU227-SER237 | |
| A | GLU299-THR390 | |
| A | ALA557-ARG565 | |
| A | THR614-ALA626 | |
| A | ARG673-MET683 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 1 |
| Details | ACT_SITE: ACT_SITE => ECO:0000255|PROSITE-ProRule:PRU10095 |
| Chain | Residue | Details |
| B | GLN224 | |
| A | ILE593-ILE613 | |
| A | PHE627-VAL649 | |
| A | VAL654-LEU672 | |
| A | MET149-ALA169 | |
| A | ALA183-PHE203 | |
| A | LEU206-PHE226 | |
| A | TRP238-ILE258 | |
| A | VAL278-PRO298 | |
| A | LEU391-PHE411 | |
| A | PHE536-SER556 | |
| A | LEU566-GLY586 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU10095, ECO:0000269|PubMed:17173035, ECO:0000305|PubMed:9783750, ECO:0007744|PDB:2NYY, ECO:0007744|PDB:2NZ9, ECO:0007744|PDB:3BTA, ECO:0007744|PDB:3QIX, ECO:0007744|PDB:3QIY, ECO:0007744|PDB:3QIZ, ECO:0007744|PDB:3QJ0 |
| Chain | Residue | Details |
| B | HIS223 | |
| B | HIS227 | |
| A | PRO259-ARG277 | |
| A | PRO412-ASP535 | |
| A | ASN587-MET592 | |
| A | GLY650-LYS653 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1 |
| Details | BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU10095, ECO:0000269|PubMed:17173035, ECO:0007744|PDB:2NYY, ECO:0007744|PDB:2NZ9, ECO:0007744|PDB:3BTA, ECO:0007744|PDB:3QIX, ECO:0007744|PDB:3QIY, ECO:0007744|PDB:3QIZ, ECO:0007744|PDB:3QJ0 |
| Chain | Residue | Details |
| B | GLU262 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 1 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:18704164, ECO:0000269|PubMed:27958736, ECO:0007744|PDB:2VU9, ECO:0007744|PDB:5TPB, ECO:0007744|PDB:5TPC |
| Chain | Residue | Details |
| B | TYR1117 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 1 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:18704164, ECO:0000269|PubMed:27958736, ECO:0000305|PubMed:14731268, ECO:0007744|PDB:2VU9, ECO:0007744|PDB:5TPB, ECO:0007744|PDB:5TPC |
| Chain | Residue | Details |
| B | GLU1203 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 2 |
| Details | SITE: Transition state stabilizer => ECO:0000305|PubMed:11827515 |
| Chain | Residue | Details |
| B | ALA363 | |
| B | PHE366 | |
| A | ASN516 |
