9F11
CryoEM structure of the F plasmid relaxosome with oriT DNA ss-27_+3ds+4_+143 and TraI its TE mode, derived from ss-27_+3ds+4_+143-R Locally-refined 3.68 A Map.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | T-strand DNA (96-MER) | polymer | 170 | 52621.7 | 1 | Escherichia coli K-12 | ||
| 2 | B (B) | R-strand DNA (85-MER) | polymer | 140 | 43132.6 | 1 | Escherichia coli K-12 | ||
| 3 | C (C) | Integration host factor subunit alpha | polymer | 99 | 11374.0 | 1 | UniProt (P0A6X7) Pfam (PF00216) | Escherichia coli K-12 | IHF-alpha |
| 4 | D (D) | Integration host factor subunit beta | polymer | 94 | 10671.2 | 1 | UniProt (P0A6Y1) Pfam (PF00216) | Escherichia coli K-12 | IHF-beta |
| 5 | E, F, G (E, F, G) | Relaxosome protein TraY | polymer | 131 | 15210.4 | 3 | UniProt (P06627) Pfam (PF05509) | Escherichia coli K-12 | |
| 6 | H (H) | Multifunctional conjugation protein TraI | polymer | 1763 | 193050.9 | 1 | UniProt (P14565) Pfam (PF07057) Pfam (PF08751) Pfam (PF22232) Pfam (PF18272) Pfam (PF18340) Pfam (PF13604) | Escherichia coli K-12 | |
| 7 | I (H) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) |
Sequence modifications
H: 1 - 1756 (UniProt: P14565)
| PDB | External Database | Details |
|---|---|---|
| Met -6 | - | initiating methionine |
| Glu -5 | - | expression tag |
| Phe -4 | - | expression tag |
| Glu -3 | - | expression tag |
| Leu -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Thr 0 | - | expression tag |
| Phe 16 | Tyr 16 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 356481.6 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 24.3 | |
| All* | Total formula weight | 356505.9 |
