9F07

TUBULIN:STATHMIN:DARPIN:TAU MTBR3 COMPLEX

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	Tubulin alpha chain	polymer	451	50204.4	2	UniProt (D0VWZ0) Pfam (PF00091) Pfam (PF03953)	Ovis aries (sheep)
2	B, F (B, F)	Tubulin beta chain	polymer	445	49999.9	2	UniProt (D0VWY9) Pfam (PF00091) Pfam (PF03953)	Ovis aries (sheep)
3	C, G (C, G)	Stathmin-4	polymer	121	13157.8	2	UniProt (Q9H169) Pfam (PF00836)	Homo sapiens (human)	Stathmin-like protein B3,RB3,SLD1-R3
4	D, H (D, H)	D2-R3	polymer	190	19666.9	2		synthetic construct
5	I, U (A, E)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	2	Chemie (GTP)
6	J, V (A, E)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
7	K, W (B, F)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	2	Chemie (GDP)
8	L, M, N, O, X... (B, F)	GLYCEROL	non-polymer		92.1	7	Chemie (GOL)
9	CA, P (F, B)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)
10	Q (B)	PENTAETHYLENE GLYCOL	non-polymer		238.3	1	Chemie (1PE)
11	R (B)	HEXAETHYLENE GLYCOL	non-polymer		282.3	1	Chemie (P6G)
12	AA, BA, DA, S, T (F, H, B, D)	TRIETHYLENE GLYCOL	non-polymer		150.2	5	Chemie (PGE)
13	EA, FA, GA, HA, IA... (A, B, C, D, E...)	water	water		18.0	328	Chemie (HOH)

Sequence modifications

A, E: 1 - 451 (UniProt: D0VWZ0)

PDB	External Database	Details
Ser 232	Gly 232	conflict
Ser 340	Thr 340	conflict

B, F: 1 - 455 (UniProt: D0VWY9)

PDB	External Database	Details
Cys 203	Ser 201	conflict
Ile 318	Val 316	conflict

C, G: 206 - 263 (UniProt: Q9H169)

PDB	External Database	Details
Met 204	-	initiating methionine
Ala 205	-	expression tag
Ala 215	Cys 58	engineered mutation
Trp 221	Phe 64	engineered mutation

C, G: 264 - 291 (UniProt: Q9H169)

PDB	External Database	Details
Phe 266	Leu 160	engineered mutation
Gly 292	-	expression tag
Gly 293	-	expression tag
Gly 294	-	expression tag
Gly 295	-	expression tag
Ser 296	-	expression tag
Gly 297	-	expression tag
Gly 298	-	expression tag
Gly 299	-	expression tag
Gly 300	-	expression tag
Ser 301	-	expression tag
Gly 302	-	expression tag
Gly 303	-	expression tag
Gly 304	-	expression tag
Ser 305	-	expression tag
Val 306	-	expression tag
Gln 307	-	expression tag
Ile 308	-	expression tag
Val 309	-	expression tag
Tyr 310	-	expression tag
Lys 311	-	expression tag
Pro 312	-	expression tag
Val 313	-	expression tag
Asp 314	-	expression tag
Leu 315	-	expression tag
Ser 316	-	expression tag
Lys 317	-	expression tag
Val 318	-	expression tag
Thr 319	-	expression tag
Ser 320	-	expression tag
Lys 321	-	expression tag
Ser 322	-	expression tag
Gly 323	-	expression tag
Ser 324	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	266058.2
Non-Polymers*	Number of molecules	22
	Total formula weight	4109.7
All*	Total formula weight	270167.9

*Water molecules are not included.